CRISPR-U™ Gene Knockout Cell Line Strategy

ASIC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ASIC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ASIC1
Gene id 41
Organism Homo sapiens
Official full symbol acid sensing ion channel subunit 1
Gene type protein-coding
Also known as ACCN2, ASIC, BNaC2
Summary This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 12
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ASIC1-201 ENST00000228468.8 4072 574aa Protein coding CCDS8796 P78348-1 - TSL:1, GENCODE basic,
ASIC1-202 ENST00000447966.7 3886 528aa Protein coding CCDS44876 P78348-2 NM_001095.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ASIC1-208 ENST00000552438.5 3327 562aa Protein coding CCDS58228 P78348-3 - TSL:1, GENCODE basic,
ASIC1-203 ENST00000453327.7 1481 396aa Protein coding - H7C1W9 - CDS 5' incomplete, TSL:2,
ASIC1-209 ENST00000552633.2 614 127aa Protein coding - H0YHD6 - CDS 5' incomplete, TSL:5,
ASIC1-206 ENST00000550558.5 3819 263aa Nonsense mediated decay - F8VSK4 - TSL:2,
ASIC1-207 ENST00000551199.1 1223 No protein Retained intron - - - TSL:2,
ASIC1-204 ENST00000548350.1 560 No protein Retained intron - - - TSL:4,
ASIC1-205 ENST00000549792.1 350 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ASIC1
This KO Strategy loading
Red Cotton™ Notes Gene ASIC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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