CRISPR-U™ Gene Knockout Cell Line Strategy

ARRB2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARRB2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARRB2
Gene id 409
Organism Homo sapiens
Official full symbol arrestin beta 2
Gene type protein-coding
Also known as ARB2, ARR2, BARR2
Summary Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 17
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARRB2-204 ENST00000412477.7 1779 430aa Protein coding CCDS58504 P32121-4 - TSL:2, GENCODE basic,
ARRB2-201 ENST00000269260.7 1778 409aa Protein coding CCDS11050 P32121-1 NM_004313.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARRB2-202 ENST00000346341.6 1769 406aa Protein coding CCDS58505 P32121-2 - TSL:2, GENCODE basic,
ARRB2-210 ENST00000572457.5 1660 217aa Protein coding CCDS82039 Q68DZ5 - TSL:5, GENCODE basic,
ARRB2-207 ENST00000571206.1 1659 217aa Protein coding CCDS82039 Q68DZ5 - TSL:5, GENCODE basic,
ARRB2-218 ENST00000575877.5 1579 330aa Protein coding CCDS59276 K7ENA6 - TSL:5, GENCODE basic,
ARRB2-216 ENST00000574954.5 1348 217aa Protein coding CCDS82039 Q68DZ5 - TSL:1, GENCODE basic,
ARRB2-203 ENST00000381488.10 1236 394aa Protein coding CCDS11051 P32121-5 - TSL:2, GENCODE basic,
ARRB2-208 ENST00000571428.5 864 113aa Protein coding - K7EL17 - CDS 3' incomplete, TSL:3,
ARRB2-219 ENST00000576235.1 991 196aa Nonsense mediated decay - I3L412 - CDS 5' incomplete, TSL:5,
ARRB2-214 ENST00000574502.5 709 64aa Nonsense mediated decay - I3L0V6 - TSL:3,
ARRB2-211 ENST00000572497.5 432 74aa Nonsense mediated decay - I3L1M6 - TSL:4,
ARRB2-205 ENST00000570718.5 564 No protein Processed transcript - - - TSL:4,
ARRB2-206 ENST00000570739.5 553 No protein Processed transcript - - - TSL:4,
ARRB2-209 ENST00000571791.5 851 No protein Retained intron - - - TSL:2,
ARRB2-213 ENST00000573886.5 665 No protein Retained intron - - - TSL:2,
ARRB2-220 ENST00000577054.1 579 No protein Retained intron - - - TSL:3,
ARRB2-217 ENST00000575131.5 550 No protein Retained intron - - - TSL:2,
ARRB2-212 ENST00000572782.1 523 No protein Retained intron - - - TSL:3,
ARRB2-215 ENST00000574888.5 509 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARRB2
This KO Strategy loading
Red Cotton™ Notes Gene ARRB2 had been KO in hepg2 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×