CRISPR-U™ Gene Knockout Cell Line
Strategy
ARRB2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARRB2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARRB2 |
| Gene id | 409 |
| Organism | Homo sapiens |
| Official full symbol | arrestin beta 2 |
| Gene type | protein-coding |
| Also known as | ARB2, ARR2, BARR2 |
| Summary | Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARRB2-204 | ENST00000412477.7 | 1779 | 430aa | Protein coding | CCDS58504 | P32121-4 | - | TSL:2, GENCODE basic, |
| ARRB2-201 | ENST00000269260.7 | 1778 | 409aa | Protein coding | CCDS11050 | P32121-1 | NM_004313.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ARRB2-202 | ENST00000346341.6 | 1769 | 406aa | Protein coding | CCDS58505 | P32121-2 | - | TSL:2, GENCODE basic, |
| ARRB2-210 | ENST00000572457.5 | 1660 | 217aa | Protein coding | CCDS82039 | Q68DZ5 | - | TSL:5, GENCODE basic, |
| ARRB2-207 | ENST00000571206.1 | 1659 | 217aa | Protein coding | CCDS82039 | Q68DZ5 | - | TSL:5, GENCODE basic, |
| ARRB2-218 | ENST00000575877.5 | 1579 | 330aa | Protein coding | CCDS59276 | K7ENA6 | - | TSL:5, GENCODE basic, |
| ARRB2-216 | ENST00000574954.5 | 1348 | 217aa | Protein coding | CCDS82039 | Q68DZ5 | - | TSL:1, GENCODE basic, |
| ARRB2-203 | ENST00000381488.10 | 1236 | 394aa | Protein coding | CCDS11051 | P32121-5 | - | TSL:2, GENCODE basic, |
| ARRB2-208 | ENST00000571428.5 | 864 | 113aa | Protein coding | - | K7EL17 | - | CDS 3' incomplete, TSL:3, |
| ARRB2-219 | ENST00000576235.1 | 991 | 196aa | Nonsense mediated decay | - | I3L412 | - | CDS 5' incomplete, TSL:5, |
| ARRB2-214 | ENST00000574502.5 | 709 | 64aa | Nonsense mediated decay | - | I3L0V6 | - | TSL:3, |
| ARRB2-211 | ENST00000572497.5 | 432 | 74aa | Nonsense mediated decay | - | I3L1M6 | - | TSL:4, |
| ARRB2-205 | ENST00000570718.5 | 564 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARRB2-206 | ENST00000570739.5 | 553 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARRB2-209 | ENST00000571791.5 | 851 | No protein | Retained intron | - | - | - | TSL:2, |
| ARRB2-213 | ENST00000573886.5 | 665 | No protein | Retained intron | - | - | - | TSL:2, |
| ARRB2-220 | ENST00000577054.1 | 579 | No protein | Retained intron | - | - | - | TSL:3, |
| ARRB2-217 | ENST00000575131.5 | 550 | No protein | Retained intron | - | - | - | TSL:2, |
| ARRB2-212 | ENST00000572782.1 | 523 | No protein | Retained intron | - | - | - | TSL:3, |
| ARRB2-215 | ENST00000574888.5 | 509 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARRB2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARRB2 had been KO in hepg2 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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