CRISPR-U™ Gene Knockout Cell Line
Strategy
LTBR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LTBR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LTBR |
| Gene id | 4055 |
| Organism | Homo sapiens |
| Official full symbol | lymphotoxin beta receptor |
| Gene type | protein-coding |
| Also known as | D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3 |
| Summary | This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LTBR-201 | ENST00000228918.9 | 2134 | 435aa | Protein coding | CCDS8544 | P36941-1 | NM_002342.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| LTBR-207 | ENST00000539925.5 | 1665 | 416aa | Protein coding | CCDS59233 | P36941-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| LTBR-209 | ENST00000541102.1 | 1084 | 292aa | Protein coding | - | F5H2E7 | - | TSL:5, GENCODE basic, |
| LTBR-206 | ENST00000536876.5 | 842 | 217aa | Protein coding | - | F5H6F8 | - | CDS 3' incomplete, TSL:5, |
| LTBR-211 | ENST00000543190.5 | 785 | 74aa | Protein coding | - | F5H1S5 | - | TSL:3, GENCODE basic, |
| LTBR-213 | ENST00000544454.5 | 632 | 148aa | Nonsense mediated decay | - | F5H627 | - | TSL:5, |
| LTBR-215 | ENST00000546296.5 | 783 | No protein | Processed transcript | - | - | - | TSL:4, |
| LTBR-210 | ENST00000542830.5 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
| LTBR-203 | ENST00000441074.3 | 2327 | No protein | Retained intron | - | - | - | TSL:1, |
| LTBR-204 | ENST00000444814.2 | 1553 | No protein | Retained intron | - | - | - | TSL:2, |
| LTBR-205 | ENST00000535739.5 | 928 | No protein | Retained intron | - | - | - | TSL:5, |
| LTBR-202 | ENST00000440421.2 | 729 | No protein | Retained intron | - | - | - | TSL:2, |
| LTBR-208 | ENST00000541005.1 | 583 | No protein | Retained intron | - | - | - | TSL:2, |
| LTBR-214 | ENST00000545445.1 | 379 | No protein | Retained intron | - | - | - | TSL:2, |
| LTBR-212 | ENST00000543542.1 | 367 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LTBR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LTBR had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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