CRISPR-U™ Gene Knockout Cell Line Strategy

LTBR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LTBR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LTBR
Gene id 4055
Organism Homo sapiens
Official full symbol lymphotoxin beta receptor
Gene type protein-coding
Summary This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Genomic regions Chromosome 12
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LTBR-201 ENST00000228918.9 2134 435aa Protein coding CCDS8544 P36941-1 NM_002342.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
LTBR-207 ENST00000539925.5 1665 416aa Protein coding CCDS59233 P36941-2 - TSL:2, GENCODE basic, APPRIS ALT2,
LTBR-209 ENST00000541102.1 1084 292aa Protein coding - F5H2E7 - TSL:5, GENCODE basic,
LTBR-206 ENST00000536876.5 842 217aa Protein coding - F5H6F8 - CDS 3' incomplete, TSL:5,
LTBR-211 ENST00000543190.5 785 74aa Protein coding - F5H1S5 - TSL:3, GENCODE basic,
LTBR-213 ENST00000544454.5 632 148aa Nonsense mediated decay - F5H627 - TSL:5,
LTBR-215 ENST00000546296.5 783 No protein Processed transcript - - - TSL:4,
LTBR-210 ENST00000542830.5 539 No protein Processed transcript - - - TSL:4,
LTBR-203 ENST00000441074.3 2327 No protein Retained intron - - - TSL:1,
LTBR-204 ENST00000444814.2 1553 No protein Retained intron - - - TSL:2,
LTBR-205 ENST00000535739.5 928 No protein Retained intron - - - TSL:5,
LTBR-202 ENST00000440421.2 729 No protein Retained intron - - - TSL:2,
LTBR-208 ENST00000541005.1 583 No protein Retained intron - - - TSL:2,
LTBR-214 ENST00000545445.1 379 No protein Retained intron - - - TSL:2,
LTBR-212 ENST00000543542.1 367 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LTBR
This KO Strategy loading
Red Cotton™ Notes Gene LTBR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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