CRISPR-U™ Gene Knockout Cell Line Strategy

LTA4H Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LTA4H Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LTA4H
Gene id 4048
Organism Homo sapiens
Official full symbol leukotriene A4 hydrolase
Gene type protein-coding
Summary The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LTA4H-201 ENST00000228740.7 2140 611aa Protein coding CCDS9059 P09960-1 NM_000895.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LTA4H-209 ENST00000552789.5 2025 587aa Protein coding CCDS58267 P09960-4 - TSL:1, GENCODE basic,
LTA4H-202 ENST00000413268.6 1908 508aa Protein coding CCDS58266 P09960-3 - TSL:2, GENCODE basic,
LTA4H-207 ENST00000548852.5 1810 132aa Nonsense mediated decay - B4DEH5 - TSL:2,
LTA4H-206 ENST00000548375.5 639 No protein Processed transcript - - - TSL:5,
LTA4H-210 ENST00000553041.5 6109 No protein Retained intron - - - TSL:2,
LTA4H-203 ENST00000537111.6 1881 No protein Retained intron - - - TSL:1,
LTA4H-205 ENST00000547982.1 986 No protein Retained intron - - - TSL:1,
LTA4H-208 ENST00000552091.1 595 No protein Retained intron - - - TSL:2,
LTA4H-204 ENST00000547393.5 543 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LTA4H
This KO Strategy loading
Red Cotton™ Notes Gene LTA4H had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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