CRISPR-U™ Gene Knockout Cell Line
Strategy
LTA4H Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LTA4H Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LTA4H |
| Gene id | 4048 |
| Organism | Homo sapiens |
| Official full symbol | leukotriene A4 hydrolase |
| Gene type | protein-coding |
| Summary | The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LTA4H-201 | ENST00000228740.7 | 2140 | 611aa | Protein coding | CCDS9059 | P09960-1 | NM_000895.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LTA4H-209 | ENST00000552789.5 | 2025 | 587aa | Protein coding | CCDS58267 | P09960-4 | - | TSL:1, GENCODE basic, |
| LTA4H-202 | ENST00000413268.6 | 1908 | 508aa | Protein coding | CCDS58266 | P09960-3 | - | TSL:2, GENCODE basic, |
| LTA4H-207 | ENST00000548852.5 | 1810 | 132aa | Nonsense mediated decay | - | B4DEH5 | - | TSL:2, |
| LTA4H-206 | ENST00000548375.5 | 639 | No protein | Processed transcript | - | - | - | TSL:5, |
| LTA4H-210 | ENST00000553041.5 | 6109 | No protein | Retained intron | - | - | - | TSL:2, |
| LTA4H-203 | ENST00000537111.6 | 1881 | No protein | Retained intron | - | - | - | TSL:1, |
| LTA4H-205 | ENST00000547982.1 | 986 | No protein | Retained intron | - | - | - | TSL:1, |
| LTA4H-208 | ENST00000552091.1 | 595 | No protein | Retained intron | - | - | - | TSL:2, |
| LTA4H-204 | ENST00000547393.5 | 543 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LTA4H |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LTA4H had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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