CRISPR-U™ Gene Knockout Cell Line Strategy

NUCKS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NUCKS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUCKS1
Gene id 404775
Organism Gallus gallus
Official full symbol nuclear casein kinase and cyclin dependent kinase substrate 1
Gene type protein-coding
Also known as JC7, NUCKS
Genomic regions Chromosome 26
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype UniProt Match Flags
NUCKS1-201 ENSGALT00000094134.1 2498 292aa Protein coding A0A3Q2UD21 APPRIS ALT2,
NUCKS1-203 ENSGALT00000102630.1 2398 355aa Protein coding A0A3Q2U1N1 APPRIS P5,
NUCKS1-202 ENSGALT00000001004.6 2107 307aa Protein coding F1N9R8 -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUCKS1
This KO Strategy loading
Red Cotton™ Notes Gene NUCKS1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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