CRISPR-U™ Gene Knockout Cell Line
Strategy
NUCKS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUCKS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUCKS1 |
| Gene id | 404775 |
| Organism | Gallus gallus |
| Official full symbol | nuclear casein kinase and cyclin dependent kinase substrate 1 |
| Gene type | protein-coding |
| Also known as | JC7, NUCKS |
| Genomic regions | Chromosome 26 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | UniProt Match | Flags |
| NUCKS1-201 | ENSGALT00000094134.1 | 2498 | 292aa | Protein coding | A0A3Q2UD21 | APPRIS ALT2, |
| NUCKS1-203 | ENSGALT00000102630.1 | 2398 | 355aa | Protein coding | A0A3Q2U1N1 | APPRIS P5, |
| NUCKS1-202 | ENSGALT00000001004.6 | 2107 | 307aa | Protein coding | F1N9R8 | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUCKS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUCKS1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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