CRISPR-U™ Gene Knockout Cell Line
Strategy
IL21 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL21 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL21 |
| Gene id | 403123 |
| Organism | Sus scrofa |
| Official full symbol | interleukin 21 |
| Gene type | protein-coding |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | UniProt Match | Flags |
| IL21-202 | ENSSSCT00000043075.2 | 962 | 160aa | Protein coding | A0A287AHG3 | APPRIS P5, |
| IL21-201 | ENSSSCT00000009953.4 | 460 | 152aa | Protein coding | F1RQN2 | APPRIS ALT2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL21 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL21 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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