CRISPR-U™ Gene Knockout Cell Line Strategy

LMO2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LMO2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LMO2
Gene id 4005
Organism Homo sapiens
Official full symbol LIM domain only 2
Gene type protein-coding
Also known as LMO-2, RBTN2, RBTNL1, RHOM2, TTG2
Summary LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LMO2-201 ENST00000257818.3 2081 227aa Protein coding CCDS7888 P25791-3 NM_005574.4 TSL:1, GENCODE basic, MANE Select v0.92,
LMO2-202 ENST00000395833.7 1687 158aa Protein coding CCDS44567 P25791-1 - TSL:1, GENCODE basic, APPRIS P1,
LMO2-203 ENST00000411482.1 1519 55aa Nonsense mediated decay - P25791-4 - TSL:1,
LMO2-206 ENST00000493667.1 390 No protein Processed transcript - - - TSL:3,
LMO2-204 ENST00000464025.5 1550 No protein Retained intron - - - TSL:1,
LMO2-205 ENST00000465614.1 869 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LMO2
This KO Strategy loading
Red Cotton™ Notes Gene LMO2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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