CRISPR-U™ Gene Knockout Cell Line Strategy

LGALS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LGALS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LGALS1
Gene id 3956
Organism Homo sapiens
Official full symbol galectin 1
Gene type protein-coding
Also known as GAL1, GBP
Summary The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.
Genomic regions Chromosome 22
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LGALS1-201 ENST00000215909.10 528 135aa Protein coding CCDS13954 A0A384MR27 P09382 NM_002305.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LGALS1-202 ENST00000425542.5 637 69aa Nonsense mediated decay - F8WCQ5 - TSL:3,
LGALS1-203 ENST00000454173.1 331 38aa Nonsense mediated decay - F8WEI7 - TSL:5,
LGALS1-207 ENST00000489315.5 516 No protein Processed transcript - - - TSL:3,
LGALS1-206 ENST00000480207.5 185 No protein Processed transcript - - - TSL:5,
LGALS1-205 ENST00000472321.1 546 No protein Retained intron - - - TSL:2,
LGALS1-204 ENST00000464120.1 303 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LGALS1
This KO Strategy loading
Red Cotton™ Notes Gene LGALS1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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