CRISPR-U™ Gene Knockout Cell Line
Strategy
LGALS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LGALS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LGALS1 |
| Gene id | 3956 |
| Organism | Homo sapiens |
| Official full symbol | galectin 1 |
| Gene type | protein-coding |
| Also known as | GAL1, GBP |
| Summary | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LGALS1-201 | ENST00000215909.10 | 528 | 135aa | Protein coding | CCDS13954 | A0A384MR27 P09382 | NM_002305.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LGALS1-202 | ENST00000425542.5 | 637 | 69aa | Nonsense mediated decay | - | F8WCQ5 | - | TSL:3, |
| LGALS1-203 | ENST00000454173.1 | 331 | 38aa | Nonsense mediated decay | - | F8WEI7 | - | TSL:5, |
| LGALS1-207 | ENST00000489315.5 | 516 | No protein | Processed transcript | - | - | - | TSL:3, |
| LGALS1-206 | ENST00000480207.5 | 185 | No protein | Processed transcript | - | - | - | TSL:5, |
| LGALS1-205 | ENST00000472321.1 | 546 | No protein | Retained intron | - | - | - | TSL:2, |
| LGALS1-204 | ENST00000464120.1 | 303 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LGALS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LGALS1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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