CRISPR-U™ Gene Knockout Cell Line Strategy

LDLR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LDLR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LDLR
Gene id 3949
Organism Homo sapiens
Official full symbol low density lipoprotein receptor
Gene type protein-coding
Also known as FH, FHC, FHCL1, LDLCQ2
Summary The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LDLR-208 ENST00000558518.6 5173 860aa Protein coding CCDS12254 P01130-1 NM_000527.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
LDLR-207 ENST00000558013.5 3144 858aa Protein coding CCDS58651 P01130-5 - TSL:1, GENCODE basic, APPRIS ALT2,
LDLR-203 ENST00000535915.5 2768 819aa Protein coding CCDS56085 P01130-4 - TSL:2, GENCODE basic,
LDLR-204 ENST00000545707.5 2429 682aa Protein coding CCDS56083 P01130-2 - TSL:2, GENCODE basic,
LDLR-202 ENST00000455727.6 2333 692aa Protein coding CCDS56084 P01130-3 - TSL:2, GENCODE basic,
LDLR-201 ENST00000252444.9 5337 945aa Protein coding - J3KMZ9 - CDS 5' incomplete, TSL:1,
LDLR-205 ENST00000557933.5 2941 948aa Protein coding - H0YMD1 - TSL:5, GENCODE basic, APPRIS ALT2,
LDLR-212 ENST00000560467.1 928 309aa Protein coding - H0YM92 - CDS 5' and 3' incomplete, TSL:3,
LDLR-210 ENST00000559340.1 605 170aa Nonsense mediated decay - H0YMQ3 - CDS 5' incomplete, TSL:5,
LDLR-214 ENST00000560628.1 535 No protein Processed transcript - - - TSL:3,
LDLR-206 ENST00000557958.1 1881 No protein Retained intron - - - TSL:2,
LDLR-213 ENST00000560502.5 509 No protein Retained intron - - - TSL:2,
LDLR-209 ENST00000558528.1 456 No protein Retained intron - - - TSL:2,
LDLR-211 ENST00000560173.1 389 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LDLR
This KO Strategy loading
Red Cotton™ Notes Gene LDLR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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