CRISPR-U™ Gene Knockout Cell Line Strategy

LDHB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LDHB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LDHB
Gene id 3945
Organism Homo sapiens
Official full symbol lactate dehydrogenase B
Gene type protein-coding
Also known as HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5
Summary This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LDHB-203 ENST00000396076.5 1538 334aa Protein coding CCDS8691 P07195 Q5U077 - TSL:5, GENCODE basic, APPRIS P1,
LDHB-201 ENST00000350669.5 1303 334aa Protein coding CCDS8691 P07195 Q5U077 NM_002300.8 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LDHB-211 ENST00000673047.2 1303 341aa Protein coding - A0A5F9ZHM4 - GENCODE basic,
LDHB-202 ENST00000396075.5 744 232aa Protein coding - A8MW50 - CDS 3' incomplete, TSL:3,
LDHB-204 ENST00000450584.5 732 136aa Protein coding - C9J7H8 - CDS 3' incomplete, TSL:3,
LDHB-208 ENST00000539782.1 487 102aa Protein coding - F5H793 - CDS 3' incomplete, TSL:3,
LDHB-207 ENST00000535112.1 252 No protein Processed transcript - - - TSL:5,
LDHB-205 ENST00000470280.1 710 No protein Retained intron - - - TSL:2,
LDHB-206 ENST00000470985.3 678 No protein Retained intron - - - TSL:1,
LDHB-209 ENST00000542765.4 672 No protein Retained intron - - - TSL:2,
LDHB-210 ENST00000544151.1 544 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LDHB
This KO Strategy loading
Red Cotton™ Notes Gene LDHB had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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