CRISPR-U™ Gene Knockout Cell Line
Strategy
LDHB Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LDHB Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LDHB |
| Gene id | 3945 |
| Organism | Homo sapiens |
| Official full symbol | lactate dehydrogenase B |
| Gene type | protein-coding |
| Also known as | HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5 |
| Summary | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LDHB-203 | ENST00000396076.5 | 1538 | 334aa | Protein coding | CCDS8691 | P07195 Q5U077 | - | TSL:5, GENCODE basic, APPRIS P1, |
| LDHB-201 | ENST00000350669.5 | 1303 | 334aa | Protein coding | CCDS8691 | P07195 Q5U077 | NM_002300.8 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LDHB-211 | ENST00000673047.2 | 1303 | 341aa | Protein coding | - | A0A5F9ZHM4 | - | GENCODE basic, |
| LDHB-202 | ENST00000396075.5 | 744 | 232aa | Protein coding | - | A8MW50 | - | CDS 3' incomplete, TSL:3, |
| LDHB-204 | ENST00000450584.5 | 732 | 136aa | Protein coding | - | C9J7H8 | - | CDS 3' incomplete, TSL:3, |
| LDHB-208 | ENST00000539782.1 | 487 | 102aa | Protein coding | - | F5H793 | - | CDS 3' incomplete, TSL:3, |
| LDHB-207 | ENST00000535112.1 | 252 | No protein | Processed transcript | - | - | - | TSL:5, |
| LDHB-205 | ENST00000470280.1 | 710 | No protein | Retained intron | - | - | - | TSL:2, |
| LDHB-206 | ENST00000470985.3 | 678 | No protein | Retained intron | - | - | - | TSL:1, |
| LDHB-209 | ENST00000542765.4 | 672 | No protein | Retained intron | - | - | - | TSL:2, |
| LDHB-210 | ENST00000544151.1 | 544 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LDHB |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LDHB had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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