CRISPR-U™ Gene Knockout Cell Line Strategy

LBR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LBR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LBR
Gene id 3930
Organism Homo sapiens
Official full symbol lamin B receptor
Gene type protein-coding
Also known as C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18
Summary The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LBR-202 ENST00000338179.6 3812 615aa Protein coding CCDS1545 Q14739 - TSL:5, GENCODE basic, APPRIS P1,
LBR-201 ENST00000272163.9 3748 615aa Protein coding CCDS1545 Q14739 NM_002296.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LBR-205 ENST00000425080.1 874 213aa Protein coding - C9JXK0 - CDS 3' incomplete, TSL:3,
LBR-203 ENST00000421383.1 736 51aa Protein coding - C9JES9 - CDS 3' incomplete, TSL:3,
LBR-209 ENST00000651341.1 2695 411aa Nonsense mediated decay - A0A494C1L1 - -
LBR-207 ENST00000487054.1 731 No protein Processed transcript - - - TSL:3,
LBR-208 ENST00000488632.1 425 No protein Processed transcript - - - TSL:3,
LBR-206 ENST00000441022.1 536 No protein Retained intron - - - TSL:2,
LBR-204 ENST00000424022.2 478 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LBR
This KO Strategy loading
Red Cotton™ Notes Gene LBR had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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