CRISPR-U™ Gene Knockout Cell Line
Strategy
LBR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LBR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LBR |
| Gene id | 3930 |
| Organism | Homo sapiens |
| Official full symbol | lamin B receptor |
| Gene type | protein-coding |
| Also known as | C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18 |
| Summary | The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LBR-202 | ENST00000338179.6 | 3812 | 615aa | Protein coding | CCDS1545 | Q14739 | - | TSL:5, GENCODE basic, APPRIS P1, |
| LBR-201 | ENST00000272163.9 | 3748 | 615aa | Protein coding | CCDS1545 | Q14739 | NM_002296.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LBR-205 | ENST00000425080.1 | 874 | 213aa | Protein coding | - | C9JXK0 | - | CDS 3' incomplete, TSL:3, |
| LBR-203 | ENST00000421383.1 | 736 | 51aa | Protein coding | - | C9JES9 | - | CDS 3' incomplete, TSL:3, |
| LBR-209 | ENST00000651341.1 | 2695 | 411aa | Nonsense mediated decay | - | A0A494C1L1 | - | - |
| LBR-207 | ENST00000487054.1 | 731 | No protein | Processed transcript | - | - | - | TSL:3, |
| LBR-208 | ENST00000488632.1 | 425 | No protein | Processed transcript | - | - | - | TSL:3, |
| LBR-206 | ENST00000441022.1 | 536 | No protein | Retained intron | - | - | - | TSL:2, |
| LBR-204 | ENST00000424022.2 | 478 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LBR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LBR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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