CRISPR-U™ Gene Knockout Cell Line
Strategy
LAMP2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMP2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAMP2 |
| Gene id | 3920 |
| Organism | Homo sapiens |
| Official full symbol | lysosomal associated membrane protein 2 |
| Gene type | protein-coding |
| Also known as | CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110 |
| Summary | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAMP2-201 | ENST00000200639.9 | 6535 | 410aa | Protein coding | CCDS14599 | P13473-1 | NM_002294.3 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| LAMP2-202 | ENST00000371335.4 | 4030 | 410aa | Protein coding | CCDS14600 | P13473-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| LAMP2-203 | ENST00000434600.6 | 2089 | 411aa | Protein coding | CCDS48159 | P13473-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| LAMP2-204 | ENST00000486593.5 | 1069 | 258aa | Nonsense mediated decay | - | H0YCG2 | - | CDS 5' incomplete, TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAMP2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAMP2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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