CRISPR-U™ Gene Knockout Cell Line Strategy

LAMP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LAMP2
Gene id 3920
Organism Homo sapiens
Official full symbol lysosomal associated membrane protein 2
Gene type protein-coding
Also known as CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110
Summary The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.
Genomic regions Chromosome X
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LAMP2-201 ENST00000200639.9 6535 410aa Protein coding CCDS14599 P13473-1 NM_002294.3 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
LAMP2-202 ENST00000371335.4 4030 410aa Protein coding CCDS14600 P13473-2 - TSL:1, GENCODE basic, APPRIS P4,
LAMP2-203 ENST00000434600.6 2089 411aa Protein coding CCDS48159 P13473-3 - TSL:1, GENCODE basic, APPRIS ALT2,
LAMP2-204 ENST00000486593.5 1069 258aa Nonsense mediated decay - H0YCG2 - CDS 5' incomplete, TSL:5,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LAMP2
This KO Strategy loading
Red Cotton™ Notes Gene LAMP2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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