CRISPR-U™ Gene Knockout Cell Line Strategy

L1CAM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human L1CAM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol L1CAM
Gene id 3897
Organism Homo sapiens
Official full symbol L1 cell adhesion molecule
Gene type protein-coding
Also known as CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1
Summary The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Genomic regions Chromosome X
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
L1CAM-205 ENST00000370060.7 5138 1257aa Protein coding CCDS14733 P32004-1 NM_001278116.2 TSL:5, GENCODE basic, APPRIS P3, MANE Select v0.92,
L1CAM-202 ENST00000361981.7 5004 1248aa Protein coding CCDS48192 P32004-3 - TSL:1, GENCODE basic, APPRIS ALT2,
L1CAM-203 ENST00000370055.5 4655 1248aa Protein coding CCDS48192 P32004-3 - TSL:5, GENCODE basic, APPRIS ALT2,
L1CAM-201 ENST00000361699.8 3888 1253aa Protein coding CCDS14734 P32004-2 - TSL:1, GENCODE basic, APPRIS ALT2,
L1CAM-204 ENST00000370058.7 692 153aa Protein coding - H3BLW5 - CDS 5' incomplete, TSL:3,
L1CAM-208 ENST00000439496.5 655 150aa Protein coding - E7EPI4 - CDS 3' incomplete, TSL:4,
L1CAM-210 ENST00000458029.1 637 83aa Protein coding - E9PHJ4 - CDS 3' incomplete, TSL:4,
L1CAM-206 ENST00000407935.6 576 128aa Protein coding - E7EVM4 - CDS 3' incomplete, TSL:3,
L1CAM-209 ENST00000455590.1 570 190aa Protein coding - H0Y5C3 - CDS 5' and 3' incomplete, TSL:4,
L1CAM-207 ENST00000420165.5 569 90aa Protein coding - E7EMY4 - CDS 3' incomplete, TSL:4,
L1CAM-216 ENST00000616195.1 740 No protein Processed transcript - - - TSL:2,
L1CAM-214 ENST00000491983.1 608 No protein Retained intron - - - TSL:3,
L1CAM-212 ENST00000474853.1 568 No protein Retained intron - - - TSL:2,
L1CAM-215 ENST00000496122.1 561 No protein Retained intron - - - TSL:4,
L1CAM-211 ENST00000460553.1 556 No protein Retained intron - - - TSL:4,
L1CAM-213 ENST00000484652.1 551 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene L1CAM
This KO Strategy loading
Red Cotton™ Notes Gene L1CAM had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

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