CRISPR-U™ Gene Knockout Cell Line
Strategy
L1CAM Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human L1CAM Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | L1CAM |
| Gene id | 3897 |
| Organism | Homo sapiens |
| Official full symbol | L1 cell adhesion molecule |
| Gene type | protein-coding |
| Also known as | CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1 |
| Summary | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| L1CAM-205 | ENST00000370060.7 | 5138 | 1257aa | Protein coding | CCDS14733 | P32004-1 | NM_001278116.2 | TSL:5, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| L1CAM-202 | ENST00000361981.7 | 5004 | 1248aa | Protein coding | CCDS48192 | P32004-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| L1CAM-203 | ENST00000370055.5 | 4655 | 1248aa | Protein coding | CCDS48192 | P32004-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| L1CAM-201 | ENST00000361699.8 | 3888 | 1253aa | Protein coding | CCDS14734 | P32004-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| L1CAM-204 | ENST00000370058.7 | 692 | 153aa | Protein coding | - | H3BLW5 | - | CDS 5' incomplete, TSL:3, |
| L1CAM-208 | ENST00000439496.5 | 655 | 150aa | Protein coding | - | E7EPI4 | - | CDS 3' incomplete, TSL:4, |
| L1CAM-210 | ENST00000458029.1 | 637 | 83aa | Protein coding | - | E9PHJ4 | - | CDS 3' incomplete, TSL:4, |
| L1CAM-206 | ENST00000407935.6 | 576 | 128aa | Protein coding | - | E7EVM4 | - | CDS 3' incomplete, TSL:3, |
| L1CAM-209 | ENST00000455590.1 | 570 | 190aa | Protein coding | - | H0Y5C3 | - | CDS 5' and 3' incomplete, TSL:4, |
| L1CAM-207 | ENST00000420165.5 | 569 | 90aa | Protein coding | - | E7EMY4 | - | CDS 3' incomplete, TSL:4, |
| L1CAM-216 | ENST00000616195.1 | 740 | No protein | Processed transcript | - | - | - | TSL:2, |
| L1CAM-214 | ENST00000491983.1 | 608 | No protein | Retained intron | - | - | - | TSL:3, |
| L1CAM-212 | ENST00000474853.1 | 568 | No protein | Retained intron | - | - | - | TSL:2, |
| L1CAM-215 | ENST00000496122.1 | 561 | No protein | Retained intron | - | - | - | TSL:4, |
| L1CAM-211 | ENST00000460553.1 | 556 | No protein | Retained intron | - | - | - | TSL:4, |
| L1CAM-213 | ENST00000484652.1 | 551 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | L1CAM |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene L1CAM had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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