CRISPR-U™ Gene Knockout Cell Line Strategy

KLC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KLC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KLC1
Gene id 3831
Organism Homo sapiens
Official full symbol kinesin light chain 1
Gene type protein-coding
Also known as KLC, KNS2, KNS2A
Summary Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.
Genomic regions Chromosome 14
Strategy Summary
This gene has 20 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KLC1-204 ENST00000348520.10 15091 573aa Protein coding CCDS41996 Q07866-1 - TSL:1, GENCODE basic,
KLC1-206 ENST00000389744.8 3250 560aa Protein coding CCDS32165 Q07866-2 - TSL:1, GENCODE basic, APPRIS ALT1,
KLC1-212 ENST00000553286.5 3135 560aa Protein coding CCDS32165 Q07866-2 - TSL:5, GENCODE basic, APPRIS ALT1,
KLC1-208 ENST00000452929.6 2453 618aa Protein coding CCDS45168 Q07866-10 - TSL:5, GENCODE basic,
KLC1-202 ENST00000334553.10 2524 639aa Protein coding - Q07866-9 - TSL:5, GENCODE basic,
KLC1-219 ENST00000555836.5 2467 630aa Protein coding - G3V5R9 - TSL:5, GENCODE basic,
KLC1-217 ENST00000554280.5 2426 609aa Protein coding - G3V3H3 - TSL:5, GENCODE basic,
KLC1-207 ENST00000445352.8 2416 549aa Protein coding - G5E9S8 - TSL:5, GENCODE basic,
KLC1-205 ENST00000380038.7 2322 576aa Protein coding - F8W6L3 - TSL:2, GENCODE basic,
KLC1-201 ENST00000246489.11 2294 637aa Protein coding - Q07866-4 - TSL:5, GENCODE basic, APPRIS ALT1,
KLC1-203 ENST00000347839.10 2271 628aa Protein coding - Q07866-6 - TSL:5, GENCODE basic, APPRIS P3,
KLC1-224 ENST00000557450.5 2265 564aa Protein coding - Q07866-3 - TSL:5, GENCODE basic,
KLC1-227 ENST00000634686.1 2229 584aa Protein coding - Q07866-7 - TSL:5, GENCODE basic,
KLC1-225 ENST00000557575.5 2188 551aa Protein coding - G3V2E7 - TSL:5, GENCODE basic,
KLC1-213 ENST00000553325.5 976 131aa Protein coding - H0YJU9 - CDS 5' incomplete, TSL:2,
KLC1-210 ENST00000537046.6 875 268aa Protein coding - H0YG16 - CDS 5' incomplete, TSL:3,
KLC1-209 ENST00000535194.2 710 60aa Protein coding - H0YGB8 - CDS 5' incomplete, TSL:3,
KLC1-214 ENST00000553436.5 630 161aa Protein coding - H0YJT3 - CDS 5' incomplete, TSL:2,
KLC1-223 ENST00000557172.5 540 150aa Protein coding - G3V2P7 - CDS 3' incomplete, TSL:4,
KLC1-220 ENST00000555856.1 535 150aa Protein coding - H0YJL0 - CDS 5' incomplete, TSL:3,
KLC1-216 ENST00000554228.5 2555 560aa Nonsense mediated decay CCDS32165 Q07866-2 - TSL:5,
KLC1-226 ENST00000557686.1 535 44aa Nonsense mediated decay - H0YJK1 - CDS 5' incomplete, TSL:3,
KLC1-221 ENST00000556986.1 1150 No protein Retained intron - - - TSL:1,
KLC1-211 ENST00000538504.6 822 No protein Retained intron - - - TSL:2,
KLC1-222 ENST00000557143.1 701 No protein Retained intron - - - TSL:2,
KLC1-218 ENST00000555467.1 559 No protein Retained intron - - - TSL:2,
KLC1-215 ENST00000553680.1 519 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KLC1
This KO Strategy loading
Red Cotton™ Notes Gene KLC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×