CRISPR-U™ Gene Knockout Cell Line
Strategy
KLC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KLC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KLC1 |
| Gene id | 3831 |
| Organism | Homo sapiens |
| Official full symbol | kinesin light chain 1 |
| Gene type | protein-coding |
| Also known as | KLC, KNS2, KNS2A |
| Summary | Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 20 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KLC1-204 | ENST00000348520.10 | 15091 | 573aa | Protein coding | CCDS41996 | Q07866-1 | - | TSL:1, GENCODE basic, |
| KLC1-206 | ENST00000389744.8 | 3250 | 560aa | Protein coding | CCDS32165 | Q07866-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| KLC1-212 | ENST00000553286.5 | 3135 | 560aa | Protein coding | CCDS32165 | Q07866-2 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| KLC1-208 | ENST00000452929.6 | 2453 | 618aa | Protein coding | CCDS45168 | Q07866-10 | - | TSL:5, GENCODE basic, |
| KLC1-202 | ENST00000334553.10 | 2524 | 639aa | Protein coding | - | Q07866-9 | - | TSL:5, GENCODE basic, |
| KLC1-219 | ENST00000555836.5 | 2467 | 630aa | Protein coding | - | G3V5R9 | - | TSL:5, GENCODE basic, |
| KLC1-217 | ENST00000554280.5 | 2426 | 609aa | Protein coding | - | G3V3H3 | - | TSL:5, GENCODE basic, |
| KLC1-207 | ENST00000445352.8 | 2416 | 549aa | Protein coding | - | G5E9S8 | - | TSL:5, GENCODE basic, |
| KLC1-205 | ENST00000380038.7 | 2322 | 576aa | Protein coding | - | F8W6L3 | - | TSL:2, GENCODE basic, |
| KLC1-201 | ENST00000246489.11 | 2294 | 637aa | Protein coding | - | Q07866-4 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| KLC1-203 | ENST00000347839.10 | 2271 | 628aa | Protein coding | - | Q07866-6 | - | TSL:5, GENCODE basic, APPRIS P3, |
| KLC1-224 | ENST00000557450.5 | 2265 | 564aa | Protein coding | - | Q07866-3 | - | TSL:5, GENCODE basic, |
| KLC1-227 | ENST00000634686.1 | 2229 | 584aa | Protein coding | - | Q07866-7 | - | TSL:5, GENCODE basic, |
| KLC1-225 | ENST00000557575.5 | 2188 | 551aa | Protein coding | - | G3V2E7 | - | TSL:5, GENCODE basic, |
| KLC1-213 | ENST00000553325.5 | 976 | 131aa | Protein coding | - | H0YJU9 | - | CDS 5' incomplete, TSL:2, |
| KLC1-210 | ENST00000537046.6 | 875 | 268aa | Protein coding | - | H0YG16 | - | CDS 5' incomplete, TSL:3, |
| KLC1-209 | ENST00000535194.2 | 710 | 60aa | Protein coding | - | H0YGB8 | - | CDS 5' incomplete, TSL:3, |
| KLC1-214 | ENST00000553436.5 | 630 | 161aa | Protein coding | - | H0YJT3 | - | CDS 5' incomplete, TSL:2, |
| KLC1-223 | ENST00000557172.5 | 540 | 150aa | Protein coding | - | G3V2P7 | - | CDS 3' incomplete, TSL:4, |
| KLC1-220 | ENST00000555856.1 | 535 | 150aa | Protein coding | - | H0YJL0 | - | CDS 5' incomplete, TSL:3, |
| KLC1-216 | ENST00000554228.5 | 2555 | 560aa | Nonsense mediated decay | CCDS32165 | Q07866-2 | - | TSL:5, |
| KLC1-226 | ENST00000557686.1 | 535 | 44aa | Nonsense mediated decay | - | H0YJK1 | - | CDS 5' incomplete, TSL:3, |
| KLC1-221 | ENST00000556986.1 | 1150 | No protein | Retained intron | - | - | - | TSL:1, |
| KLC1-211 | ENST00000538504.6 | 822 | No protein | Retained intron | - | - | - | TSL:2, |
| KLC1-222 | ENST00000557143.1 | 701 | No protein | Retained intron | - | - | - | TSL:2, |
| KLC1-218 | ENST00000555467.1 | 559 | No protein | Retained intron | - | - | - | TSL:2, |
| KLC1-215 | ENST00000553680.1 | 519 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KLC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KLC1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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