CRISPR-U™ Gene Knockout Cell Line Strategy

ARF5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARF5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARF5
Gene id 381
Organism Homo sapiens
Official full symbol ADP ribosylation factor 5
Gene type protein-coding
Summary This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARF5-201 ENST00000000233.10 1032 180aa Protein coding CCDS34745 A4D0Z3 P84085 NM_001662.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARF5-202 ENST00000415666.1 546 150aa Protein coding - C9J1Z8 - CDS 3' incomplete, TSL:5,
ARF5-205 ENST00000467281.5 567 No protein Processed transcript - - - TSL:4,
ARF5-204 ENST00000463733.5 1509 No protein Retained intron - - - TSL:5,
ARF5-206 ENST00000489673.1 566 No protein Retained intron - - - TSL:2,
ARF5-203 ENST00000459680.1 466 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARF5
This KO Strategy loading
Red Cotton™ Notes Gene ARF5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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