CRISPR-U™ Gene Knockout Cell Line
Strategy
ARF5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARF5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARF5 |
| Gene id | 381 |
| Organism | Homo sapiens |
| Official full symbol | ADP ribosylation factor 5 |
| Gene type | protein-coding |
| Summary | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARF5-201 | ENST00000000233.10 | 1032 | 180aa | Protein coding | CCDS34745 | A4D0Z3 P84085 | NM_001662.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ARF5-202 | ENST00000415666.1 | 546 | 150aa | Protein coding | - | C9J1Z8 | - | CDS 3' incomplete, TSL:5, |
| ARF5-205 | ENST00000467281.5 | 567 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARF5-204 | ENST00000463733.5 | 1509 | No protein | Retained intron | - | - | - | TSL:5, |
| ARF5-206 | ENST00000489673.1 | 566 | No protein | Retained intron | - | - | - | TSL:2, |
| ARF5-203 | ENST00000459680.1 | 466 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARF5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARF5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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