CRISPR-U™ Gene Knockout Cell Line Strategy

KIFC3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KIFC3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KIFC3
Gene id 3801
Organism Homo sapiens
Official full symbol kinesin family member C3
Gene type protein-coding
Summary This gene encodes a member of the kinesin-14 family of microtubule motors. Members of this family play a role in the formation, maintenance and remodeling of the bipolar mitotic spindle. The protein encoded by this gene has cytoplasmic functions in the interphase cells. It may also be involved in the final stages of cytokinesis. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 16
Strategy Summary
This gene has 24 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KIFC3-201 ENST00000379655.8 3427 833aa Protein coding CCDS10789 Q9BVG8-3 - TSL:1, GENCODE basic, APPRIS P4,
KIFC3-203 ENST00000445690.7 3359 826aa Protein coding CCDS45493 Q9BVG8-2 NM_001130100.2 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
KIFC3-204 ENST00000465878.6 3225 687aa Protein coding CCDS45494 Q9BVG8-5 - TSL:1, GENCODE basic, APPRIS ALT2,
KIFC3-211 ENST00000562903.5 3221 687aa Protein coding CCDS45494 Q9BVG8-5 - TSL:1, GENCODE basic, APPRIS ALT2,
KIFC3-202 ENST00000421376.6 3128 687aa Protein coding CCDS45494 Q9BVG8-5 - TSL:2, GENCODE basic, APPRIS ALT2,
KIFC3-206 ENST00000541240.5 3047 848aa Protein coding CCDS81989 Q9BVG8-6 - TSL:2, GENCODE basic, APPRIS ALT2,
KIFC3-207 ENST00000543930.5 3018 684aa Protein coding CCDS81987 B7Z896 - TSL:2, GENCODE basic, APPRIS ALT2,
KIFC3-205 ENST00000540079.6 2781 724aa Protein coding CCDS81988 F5H3M2 - TSL:2, GENCODE basic, APPRIS ALT2,
KIFC3-219 ENST00000565270.1 1081 280aa Protein coding - H3BTL6 - CDS 3' incomplete, TSL:5,
KIFC3-221 ENST00000565397.1 810 196aa Protein coding - H3BUC0 - CDS 5' incomplete, TSL:3,
KIFC3-222 ENST00000565481.5 788 147aa Protein coding - H3BTU1 - CDS 3' incomplete, TSL:5,
KIFC3-209 ENST00000562311.5 777 70aa Protein coding - H3BMR0 - CDS 3' incomplete, TSL:5,
KIFC3-210 ENST00000562503.5 737 167aa Protein coding - H3BMQ3 - CDS 3' incomplete, TSL:4,
KIFC3-234 ENST00000569619.5 698 5aa Protein coding - - - CDS 3' incomplete, TSL:3,
KIFC3-213 ENST00000563028.1 681 154aa Protein coding - H3BRQ1 - CDS 3' incomplete, TSL:4,
KIFC3-226 ENST00000566648.5 651 190aa Protein coding - H3BSN2 - CDS 3' incomplete, TSL:3,
KIFC3-223 ENST00000565684.5 623 132aa Protein coding - H3BPE2 - CDS 3' incomplete, TSL:5,
KIFC3-208 ENST00000561524.5 577 88aa Protein coding - H3BMZ5 - CDS 3' incomplete, TSL:4,
KIFC3-232 ENST00000569112.5 564 60aa Protein coding - H3BTN1 - CDS 3' incomplete, TSL:4,
KIFC3-212 ENST00000562984.5 551 59aa Protein coding - H3BMJ1 - CDS 3' incomplete, TSL:4,
KIFC3-229 ENST00000567059.5 509 165aa Protein coding - H3BTE9 - CDS 3' incomplete, TSL:3,
KIFC3-233 ENST00000569222.5 443 104aa Protein coding - H3BV47 - CDS 3' incomplete, TSL:3,
KIFC3-218 ENST00000564891.1 424 105aa Protein coding - H3BNZ0 - CDS 3' incomplete, TSL:5,
KIFC3-220 ENST00000565351.5 297 5aa Protein coding - - - CDS 3' incomplete, TSL:1,
KIFC3-216 ENST00000564136.5 3071 498aa Nonsense mediated decay - A0A0C4DGM9 - TSL:2,
KIFC3-228 ENST00000566975.5 580 No protein Processed transcript - - - TSL:4,
KIFC3-214 ENST00000563266.1 568 No protein Processed transcript - - - TSL:2,
KIFC3-215 ENST00000563295.1 558 No protein Processed transcript - - - TSL:5,
KIFC3-231 ENST00000567479.1 534 No protein Processed transcript - - - TSL:4,
KIFC3-230 ENST00000567204.5 516 No protein Processed transcript - - - TSL:4,
KIFC3-225 ENST00000565871.1 461 No protein Processed transcript - - - TSL:4,
KIFC3-227 ENST00000566914.1 724 No protein Retained intron - - - TSL:3,
KIFC3-217 ENST00000564204.1 558 No protein Retained intron - - - TSL:4,
KIFC3-224 ENST00000565753.1 473 No protein Retained intron - - - TSL:5,
KIFC3-235 ENST00000623271.1 2393 No protein TEC - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KIFC3
This KO Strategy loading
Red Cotton™ Notes Gene KIFC3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×