CRISPR-U™ Gene Knockout Cell Line
Strategy
KIFC3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KIFC3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KIFC3 |
| Gene id | 3801 |
| Organism | Homo sapiens |
| Official full symbol | kinesin family member C3 |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the kinesin-14 family of microtubule motors. Members of this family play a role in the formation, maintenance and remodeling of the bipolar mitotic spindle. The protein encoded by this gene has cytoplasmic functions in the interphase cells. It may also be involved in the final stages of cytokinesis. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 24 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KIFC3-201 | ENST00000379655.8 | 3427 | 833aa | Protein coding | CCDS10789 | Q9BVG8-3 | - | TSL:1, GENCODE basic, APPRIS P4, |
| KIFC3-203 | ENST00000445690.7 | 3359 | 826aa | Protein coding | CCDS45493 | Q9BVG8-2 | NM_001130100.2 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| KIFC3-204 | ENST00000465878.6 | 3225 | 687aa | Protein coding | CCDS45494 | Q9BVG8-5 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| KIFC3-211 | ENST00000562903.5 | 3221 | 687aa | Protein coding | CCDS45494 | Q9BVG8-5 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| KIFC3-202 | ENST00000421376.6 | 3128 | 687aa | Protein coding | CCDS45494 | Q9BVG8-5 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| KIFC3-206 | ENST00000541240.5 | 3047 | 848aa | Protein coding | CCDS81989 | Q9BVG8-6 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| KIFC3-207 | ENST00000543930.5 | 3018 | 684aa | Protein coding | CCDS81987 | B7Z896 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| KIFC3-205 | ENST00000540079.6 | 2781 | 724aa | Protein coding | CCDS81988 | F5H3M2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| KIFC3-219 | ENST00000565270.1 | 1081 | 280aa | Protein coding | - | H3BTL6 | - | CDS 3' incomplete, TSL:5, |
| KIFC3-221 | ENST00000565397.1 | 810 | 196aa | Protein coding | - | H3BUC0 | - | CDS 5' incomplete, TSL:3, |
| KIFC3-222 | ENST00000565481.5 | 788 | 147aa | Protein coding | - | H3BTU1 | - | CDS 3' incomplete, TSL:5, |
| KIFC3-209 | ENST00000562311.5 | 777 | 70aa | Protein coding | - | H3BMR0 | - | CDS 3' incomplete, TSL:5, |
| KIFC3-210 | ENST00000562503.5 | 737 | 167aa | Protein coding | - | H3BMQ3 | - | CDS 3' incomplete, TSL:4, |
| KIFC3-234 | ENST00000569619.5 | 698 | 5aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
| KIFC3-213 | ENST00000563028.1 | 681 | 154aa | Protein coding | - | H3BRQ1 | - | CDS 3' incomplete, TSL:4, |
| KIFC3-226 | ENST00000566648.5 | 651 | 190aa | Protein coding | - | H3BSN2 | - | CDS 3' incomplete, TSL:3, |
| KIFC3-223 | ENST00000565684.5 | 623 | 132aa | Protein coding | - | H3BPE2 | - | CDS 3' incomplete, TSL:5, |
| KIFC3-208 | ENST00000561524.5 | 577 | 88aa | Protein coding | - | H3BMZ5 | - | CDS 3' incomplete, TSL:4, |
| KIFC3-232 | ENST00000569112.5 | 564 | 60aa | Protein coding | - | H3BTN1 | - | CDS 3' incomplete, TSL:4, |
| KIFC3-212 | ENST00000562984.5 | 551 | 59aa | Protein coding | - | H3BMJ1 | - | CDS 3' incomplete, TSL:4, |
| KIFC3-229 | ENST00000567059.5 | 509 | 165aa | Protein coding | - | H3BTE9 | - | CDS 3' incomplete, TSL:3, |
| KIFC3-233 | ENST00000569222.5 | 443 | 104aa | Protein coding | - | H3BV47 | - | CDS 3' incomplete, TSL:3, |
| KIFC3-218 | ENST00000564891.1 | 424 | 105aa | Protein coding | - | H3BNZ0 | - | CDS 3' incomplete, TSL:5, |
| KIFC3-220 | ENST00000565351.5 | 297 | 5aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:1, |
| KIFC3-216 | ENST00000564136.5 | 3071 | 498aa | Nonsense mediated decay | - | A0A0C4DGM9 | - | TSL:2, |
| KIFC3-228 | ENST00000566975.5 | 580 | No protein | Processed transcript | - | - | - | TSL:4, |
| KIFC3-214 | ENST00000563266.1 | 568 | No protein | Processed transcript | - | - | - | TSL:2, |
| KIFC3-215 | ENST00000563295.1 | 558 | No protein | Processed transcript | - | - | - | TSL:5, |
| KIFC3-231 | ENST00000567479.1 | 534 | No protein | Processed transcript | - | - | - | TSL:4, |
| KIFC3-230 | ENST00000567204.5 | 516 | No protein | Processed transcript | - | - | - | TSL:4, |
| KIFC3-225 | ENST00000565871.1 | 461 | No protein | Processed transcript | - | - | - | TSL:4, |
| KIFC3-227 | ENST00000566914.1 | 724 | No protein | Retained intron | - | - | - | TSL:3, |
| KIFC3-217 | ENST00000564204.1 | 558 | No protein | Retained intron | - | - | - | TSL:4, |
| KIFC3-224 | ENST00000565753.1 | 473 | No protein | Retained intron | - | - | - | TSL:5, |
| KIFC3-235 | ENST00000623271.1 | 2393 | No protein | TEC | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KIFC3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KIFC3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Single gRNA lentivirus
Work flow
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