CRISPR-U™ Gene Knockout Cell Line Strategy

KHK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KHK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KHK
Gene id 3795
Organism Homo sapiens
Official full symbol ketohexokinase
Gene type protein-coding
Summary This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KHK-202 ENST00000260599.10 2411 298aa Protein coding CCDS1734 P50053-2 - TSL:1, GENCODE basic, APPRIS ALT1,
KHK-201 ENST00000260598.10 2397 298aa Protein coding CCDS1735 P50053-1 NM_006488.3 TSL:2, GENCODE basic, APPRIS P2, MANE Select v0.92,
KHK-203 ENST00000429697.1 1303 279aa Protein coding - C9JDL1 - CDS 3' incomplete, TSL:5,
KHK-206 ENST00000490823.5 1396 No protein Processed transcript - - - TSL:5,
KHK-204 ENST00000464371.1 1042 No protein Retained intron - - - TSL:1,
KHK-205 ENST00000469936.5 553 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KHK
This KO Strategy loading
Red Cotton™ Notes Gene KHK had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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