CRISPR-U™ Gene Knockout Cell Line
Strategy
KHK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KHK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KHK |
| Gene id | 3795 |
| Organism | Homo sapiens |
| Official full symbol | ketohexokinase |
| Gene type | protein-coding |
| Summary | This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KHK-202 | ENST00000260599.10 | 2411 | 298aa | Protein coding | CCDS1734 | P50053-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| KHK-201 | ENST00000260598.10 | 2397 | 298aa | Protein coding | CCDS1735 | P50053-1 | NM_006488.3 | TSL:2, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| KHK-203 | ENST00000429697.1 | 1303 | 279aa | Protein coding | - | C9JDL1 | - | CDS 3' incomplete, TSL:5, |
| KHK-206 | ENST00000490823.5 | 1396 | No protein | Processed transcript | - | - | - | TSL:5, |
| KHK-204 | ENST00000464371.1 | 1042 | No protein | Retained intron | - | - | - | TSL:1, |
| KHK-205 | ENST00000469936.5 | 553 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KHK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KHK had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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