CRISPR-U™ Gene Knockout Cell Line Strategy

KCNQ3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KCNQ3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KCNQ3
Gene id 3786
Organism Homo sapiens
Official full symbol potassium voltage-gated channel subfamily Q member 3
Gene type protein-coding
Also known as BFNC2, EBN2, KV7.3
Summary This gene encodes a protein that functions in the regulation of neuronal excitability. The encoded protein forms an M-channel by associating with the products of the related KCNQ2 or KCNQ5 genes, which both encode integral membrane proteins. M-channel currents are inhibited by M1 muscarinic acetylcholine receptors and are activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal type 2 (EBN2). Alternative splicing of this gene results in multiple transcript variants.
Genomic regions Chromosome 8
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KCNQ3-201 ENST00000388996.10 11583 872aa Protein coding CCDS34943 O43525-1 NM_004519.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
KCNQ3-204 ENST00000521134.6 2577 752aa Protein coding CCDS56554 O43525-2 - TSL:2, GENCODE basic, APPRIS ALT2,
KCNQ3-205 ENST00000621976.1 10659 751aa Protein coding - A0A087WZB4 - TSL:5, GENCODE basic,
KCNQ3-202 ENST00000519445.5 2801 860aa Protein coding - E7ET42 - TSL:5, GENCODE basic, APPRIS ALT2,
KCNQ3-206 ENST00000638588.1 2190 716aa Protein coding - A0A1W2PQ71 - CDS 3' incomplete, TSL:5,
KCNQ3-208 ENST00000639496.1 4943 319aa Nonsense mediated decay - A0A1W2PNZ2 - TSL:5,
KCNQ3-207 ENST00000639358.1 822 89aa Nonsense mediated decay - A0A1W2PRN8 - CDS 5' incomplete, TSL:5,
KCNQ3-203 ENST00000519589.1 3464 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KCNQ3
This KO Strategy loading
Red Cotton™ Notes Gene KCNQ3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×