CRISPR-U™ Gene Knockout Cell Line Strategy

KCNQ1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KCNQ1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KCNQ1
Gene id 3784
Organism Homo sapiens
Official full symbol potassium voltage-gated channel subfamily Q member 1
Gene type protein-coding
Also known as ATFB1, ATFB3, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS
Summary This gene encodes a voltage-gated potassium channel required for repolarization phase of the cardiac action potential. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. This gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KCNQ1-201 ENST00000155840.12 3224 676aa Protein coding CCDS7736 P51787-1 NM_000218.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
KCNQ1-202 ENST00000335475.6 2907 549aa Protein coding - P51787-2 - TSL:1, GENCODE basic, APPRIS ALT2,
KCNQ1-207 ENST00000646564.1 1375 310aa Protein coding - A0A2R8YEQ9 - CDS 5' incomplete,
KCNQ1-206 ENST00000526095.2 828 144aa Protein coding - A0A2R8YDV1 - TSL:3, GENCODE basic,
KCNQ1-205 ENST00000496887.6 582 193aa Protein coding - E9PPZ0 - CDS 3' incomplete, TSL:5,
KCNQ1-204 ENST00000380776.4 229 74aa Protein coding - F8W824 - CDS 3' incomplete, TSL:5,
KCNQ1-203 ENST00000345015.4 304 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KCNQ1
This KO Strategy loading
Red Cotton™ Notes Gene KCNQ1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×