CRISPR-U™ Gene Knockout Cell Line
Strategy
KCNQ1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KCNQ1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KCNQ1 |
| Gene id | 3784 |
| Organism | Homo sapiens |
| Official full symbol | potassium voltage-gated channel subfamily Q member 1 |
| Gene type | protein-coding |
| Also known as | ATFB1, ATFB3, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS |
| Summary | This gene encodes a voltage-gated potassium channel required for repolarization phase of the cardiac action potential. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. This gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KCNQ1-201 | ENST00000155840.12 | 3224 | 676aa | Protein coding | CCDS7736 | P51787-1 | NM_000218.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| KCNQ1-202 | ENST00000335475.6 | 2907 | 549aa | Protein coding | - | P51787-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| KCNQ1-207 | ENST00000646564.1 | 1375 | 310aa | Protein coding | - | A0A2R8YEQ9 | - | CDS 5' incomplete, |
| KCNQ1-206 | ENST00000526095.2 | 828 | 144aa | Protein coding | - | A0A2R8YDV1 | - | TSL:3, GENCODE basic, |
| KCNQ1-205 | ENST00000496887.6 | 582 | 193aa | Protein coding | - | E9PPZ0 | - | CDS 3' incomplete, TSL:5, |
| KCNQ1-204 | ENST00000380776.4 | 229 | 74aa | Protein coding | - | F8W824 | - | CDS 3' incomplete, TSL:5, |
| KCNQ1-203 | ENST00000345015.4 | 304 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KCNQ1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KCNQ1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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