CRISPR-U™ Gene Knockout Cell Line Strategy

ARF3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARF3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARF3
Gene id 377
Organism Homo sapiens
Official full symbol ADP ribosylation factor 3
Gene type protein-coding
Summary ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARF3-201 ENST00000256682.9 4041 181aa Protein coding CCDS8774 P61204-1 NM_001659.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARF3-208 ENST00000541959.5 807 181aa Protein coding CCDS8774 P61204-1 - TSL:5, GENCODE basic, APPRIS P1,
ARF3-202 ENST00000447318.6 1089 144aa Protein coding - P61204-2 - TSL:2, GENCODE basic,
ARF3-206 ENST00000539611.1 576 50aa Protein coding - F5H6T5 - CDS 3' incomplete, TSL:5,
ARF3-210 ENST00000545855.1 557 40aa Protein coding - F5H1V1 - CDS 3' incomplete, TSL:2,
ARF3-207 ENST00000541236.5 485 102aa Protein coding - F5H0C7 - CDS 3' incomplete, TSL:2,
ARF3-209 ENST00000541967.1 569 No protein Processed transcript - - - TSL:4,
ARF3-204 ENST00000485410.5 563 No protein Processed transcript - - - TSL:3,
ARF3-205 ENST00000536283.1 537 No protein Processed transcript - - - TSL:4,
ARF3-203 ENST00000472621.1 526 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARF3
This KO Strategy loading
Red Cotton™ Notes Gene ARF3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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