CRISPR-U™ Gene Knockout Cell Line
Strategy
ARF1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARF1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARF1 |
| Gene id | 375 |
| Organism | Homo sapiens |
| Official full symbol | ADP ribosylation factor 1 |
| Gene type | protein-coding |
| Also known as | PVNH8 |
| Summary | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARF1-214 | ENST00000541182.1 | 2006 | 181aa | Protein coding | CCDS1565 | A0A024R3Q0 P84077 | - | TSL:2, GENCODE basic, APPRIS P1, |
| ARF1-213 | ENST00000540651.5 | 1972 | 181aa | Protein coding | CCDS1565 | A0A024R3Q0 P84077 | - | TSL:2, GENCODE basic, APPRIS P1, |
| ARF1-201 | ENST00000272102.10 | 1840 | 181aa | Protein coding | CCDS1565 | A0A024R3Q0 P84077 | NM_001658.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ARF1-209 | ENST00000478424.5 | 1059 | No protein | Processed transcript | - | - | - | TSL:2, |
| ARF1-208 | ENST00000478336.5 | 930 | No protein | Processed transcript | - | - | - | TSL:2, |
| ARF1-211 | ENST00000490705.1 | 881 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARF1-203 | ENST00000470558.5 | 820 | No protein | Processed transcript | - | - | - | TSL:2, |
| ARF1-207 | ENST00000477451.5 | 789 | No protein | Processed transcript | - | - | - | TSL:5, |
| ARF1-204 | ENST00000470670.5 | 712 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARF1-202 | ENST00000469235.5 | 678 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARF1-212 | ENST00000497165.5 | 675 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARF1-210 | ENST00000482962.5 | 597 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARF1-206 | ENST00000473949.5 | 578 | No protein | Processed transcript | - | - | - | TSL:5, |
| ARF1-205 | ENST00000473546.1 | 291 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARF1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARF1 had been KO in mcf7 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$49
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$599
Single gRNA lentivirus
Work flow
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