CRISPR-U™ Gene Knockout Cell Line
Strategy
KIF7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KIF7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KIF7 |
| Gene id | 374654 |
| Organism | Homo sapiens |
| Official full symbol | kinesin family member 7 |
| Gene type | protein-coding |
| Also known as | ACLS, AGBK, HLS2, JBTS12, UNQ340 |
| Summary | This gene encodes a cilia-associated protein belonging to the kinesin family. This protein plays a role in the sonic hedgehog (SHH) signaling pathway through the regulation of GLI transcription factors. It functions as a negative regulator of the SHH pathway by preventing inappropriate activation of GLI2 in the absence of ligand, and as a positive regulator by preventing the processing of GLI3 into its repressor form. Mutations in this gene have been associated with various ciliopathies. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KIF7-201 | ENST00000394412.8 | 4567 | 1343aa | Protein coding | CCDS32325 | Q2M1P5 | NM_198525.3 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| KIF7-202 | ENST00000445906.1 | 1375 | 147aa | Nonsense mediated decay | - | F8WD21 | - | TSL:1, |
| KIF7-203 | ENST00000558928.1 | 527 | 90aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, TSL:3, |
| KIF7-204 | ENST00000677187.1 | 2074 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KIF7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KIF7 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: