CRISPR-U™ Gene Knockout Cell Line Strategy

KIF7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KIF7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KIF7
Gene id 374654
Organism Homo sapiens
Official full symbol kinesin family member 7
Gene type protein-coding
Also known as ACLS, AGBK, HLS2, JBTS12, UNQ340
Summary This gene encodes a cilia-associated protein belonging to the kinesin family. This protein plays a role in the sonic hedgehog (SHH) signaling pathway through the regulation of GLI transcription factors. It functions as a negative regulator of the SHH pathway by preventing inappropriate activation of GLI2 in the absence of ligand, and as a positive regulator by preventing the processing of GLI3 into its repressor form. Mutations in this gene have been associated with various ciliopathies.
Genomic regions Chromosome 15
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KIF7-201 ENST00000394412.8 4567 1343aa Protein coding CCDS32325 Q2M1P5 NM_198525.3 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
KIF7-202 ENST00000445906.1 1375 147aa Nonsense mediated decay - F8WD21 - TSL:1,
KIF7-203 ENST00000558928.1 527 90aa Nonsense mediated decay - - - CDS 5' incomplete, TSL:3,
KIF7-204 ENST00000677187.1 2074 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KIF7
This KO Strategy loading
Red Cotton™ Notes Gene KIF7 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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