CRISPR-U™ Gene Knockout Cell Line
Strategy
GSTK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GSTK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GSTK1 |
| Gene id | 373156 |
| Organism | Homo sapiens |
| Official full symbol | glutathione S-transferase kappa 1 |
| Gene type | protein-coding |
| Also known as | GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1 |
| Summary | This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GSTK1-207 | ENST00000479303.1 | 1185 | 282aa | Protein coding | CCDS47730 | Q9Y2Q3-2 | - | TSL:1, GENCODE basic, |
| GSTK1-201 | ENST00000358406.10 | 1032 | 226aa | Protein coding | CCDS5877 | Q9Y2Q3-1 | NM_015917.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GSTK1-202 | ENST00000409500.7 | 888 | 214aa | Protein coding | CCDS47731 | Q9Y2Q3-3 | - | TSL:2, GENCODE basic, |
| GSTK1-205 | ENST00000443571.6 | 850 | 183aa | Protein coding | CCDS47732 | Q9Y2Q3-4 | - | TSL:2, GENCODE basic, |
| GSTK1-203 | ENST00000436038.5 | 556 | 118aa | Protein coding | - | C9JNT3 | - | CDS 3' incomplete, TSL:5, |
| GSTK1-204 | ENST00000442394.5 | 2219 | 190aa | Nonsense mediated decay | - | E9PFN5 | - | TSL:5, |
| GSTK1-209 | ENST00000494735.1 | 832 | No protein | Processed transcript | - | - | - | TSL:2, |
| GSTK1-206 | ENST00000473649.1 | 2692 | No protein | Retained intron | - | - | - | TSL:2, |
| GSTK1-208 | ENST00000489654.1 | 875 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GSTK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GSTK1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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