CRISPR-U™ Gene Knockout Cell Line Strategy

GSTK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GSTK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GSTK1
Gene id 373156
Organism Homo sapiens
Official full symbol glutathione S-transferase kappa 1
Gene type protein-coding
Also known as GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1
Summary This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 7
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GSTK1-207 ENST00000479303.1 1185 282aa Protein coding CCDS47730 Q9Y2Q3-2 - TSL:1, GENCODE basic,
GSTK1-201 ENST00000358406.10 1032 226aa Protein coding CCDS5877 Q9Y2Q3-1 NM_015917.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GSTK1-202 ENST00000409500.7 888 214aa Protein coding CCDS47731 Q9Y2Q3-3 - TSL:2, GENCODE basic,
GSTK1-205 ENST00000443571.6 850 183aa Protein coding CCDS47732 Q9Y2Q3-4 - TSL:2, GENCODE basic,
GSTK1-203 ENST00000436038.5 556 118aa Protein coding - C9JNT3 - CDS 3' incomplete, TSL:5,
GSTK1-204 ENST00000442394.5 2219 190aa Nonsense mediated decay - E9PFN5 - TSL:5,
GSTK1-209 ENST00000494735.1 832 No protein Processed transcript - - - TSL:2,
GSTK1-206 ENST00000473649.1 2692 No protein Retained intron - - - TSL:2,
GSTK1-208 ENST00000489654.1 875 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GSTK1
This KO Strategy loading
Red Cotton™ Notes Gene GSTK1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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