CRISPR-U™ Gene Knockout Cell Line Strategy

JUP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human JUP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol JUP
Gene id 3728
Organism Homo sapiens
Official full symbol junction plakoglobin
Gene type protein-coding
Also known as CTNNG, DP3, DPIII, PDGB, PKGB
Summary This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.
Genomic regions Chromosome 17
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
JUP-203 ENST00000393931.8 3505 745aa Protein coding CCDS11407 A0A0S2Z487 P14923 NM_002230.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
JUP-202 ENST00000393930.5 3298 745aa Protein coding CCDS11407 A0A0S2Z487 P14923 - TSL:5, GENCODE basic, APPRIS P1,
JUP-201 ENST00000310706.9 3200 745aa Protein coding CCDS11407 A0A0S2Z487 P14923 - TSL:1, GENCODE basic, APPRIS P1,
JUP-208 ENST00000449889.5 1069 297aa Protein coding - C9JKY1 - CDS 3' incomplete, TSL:3,
JUP-206 ENST00000437187.5 1029 295aa Protein coding - C9JK18 - CDS 3' incomplete, TSL:3,
JUP-205 ENST00000424457.5 949 235aa Protein coding - C9JTX4 - CDS 3' incomplete, TSL:3,
JUP-204 ENST00000420370.5 915 276aa Protein coding - C9J826 - CDS 3' incomplete, TSL:3,
JUP-207 ENST00000437369.5 586 86aa Protein coding - C9JPI2 - CDS 3' incomplete, TSL:3,
JUP-212 ENST00000591690.5 583 104aa Protein coding - K7ERP3 - CDS 3' incomplete, TSL:3,
JUP-209 ENST00000465293.1 432 2aa Protein coding - - - CDS 3' incomplete, TSL:5,
JUP-210 ENST00000585793.1 598 No protein Retained intron - - - TSL:2,
JUP-211 ENST00000589036.1 566 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene JUP
This KO Strategy loading
Red Cotton™ Notes Gene JUP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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