CRISPR-U™ Gene Knockout Cell Line
Strategy
JUP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human JUP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | JUP |
| Gene id | 3728 |
| Organism | Homo sapiens |
| Official full symbol | junction plakoglobin |
| Gene type | protein-coding |
| Also known as | CTNNG, DP3, DPIII, PDGB, PKGB |
| Summary | This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| JUP-203 | ENST00000393931.8 | 3505 | 745aa | Protein coding | CCDS11407 | A0A0S2Z487 P14923 | NM_002230.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| JUP-202 | ENST00000393930.5 | 3298 | 745aa | Protein coding | CCDS11407 | A0A0S2Z487 P14923 | - | TSL:5, GENCODE basic, APPRIS P1, |
| JUP-201 | ENST00000310706.9 | 3200 | 745aa | Protein coding | CCDS11407 | A0A0S2Z487 P14923 | - | TSL:1, GENCODE basic, APPRIS P1, |
| JUP-208 | ENST00000449889.5 | 1069 | 297aa | Protein coding | - | C9JKY1 | - | CDS 3' incomplete, TSL:3, |
| JUP-206 | ENST00000437187.5 | 1029 | 295aa | Protein coding | - | C9JK18 | - | CDS 3' incomplete, TSL:3, |
| JUP-205 | ENST00000424457.5 | 949 | 235aa | Protein coding | - | C9JTX4 | - | CDS 3' incomplete, TSL:3, |
| JUP-204 | ENST00000420370.5 | 915 | 276aa | Protein coding | - | C9J826 | - | CDS 3' incomplete, TSL:3, |
| JUP-207 | ENST00000437369.5 | 586 | 86aa | Protein coding | - | C9JPI2 | - | CDS 3' incomplete, TSL:3, |
| JUP-212 | ENST00000591690.5 | 583 | 104aa | Protein coding | - | K7ERP3 | - | CDS 3' incomplete, TSL:3, |
| JUP-209 | ENST00000465293.1 | 432 | 2aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:5, |
| JUP-210 | ENST00000585793.1 | 598 | No protein | Retained intron | - | - | - | TSL:2, |
| JUP-211 | ENST00000589036.1 | 566 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | JUP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene JUP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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