CRISPR-U™ Gene Knockout Cell Line
Strategy
JAG2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human JAG2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | JAG2 |
| Gene id | 3714 |
| Organism | Homo sapiens |
| Official full symbol | jagged canonical Notch ligand 2 |
| Gene type | protein-coding |
| Also known as | HJ2, SER2 |
| Summary | The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| JAG2-201 | ENST00000331782.8 | 5773 | 1238aa | Protein coding | CCDS9998 | Q9Y219-1 | NM_002226.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| JAG2-202 | ENST00000347004.2 | 5720 | 1200aa | Protein coding | CCDS9999 | Q9Y219-2 | - | TSL:1, GENCODE basic, |
| JAG2-203 | ENST00000546616.1 | 2275 | No protein | Retained intron | - | - | - | TSL:5, |
| JAG2-205 | ENST00000553244.1 | 866 | No protein | Retained intron | - | - | - | TSL:3, |
| JAG2-204 | ENST00000553051.1 | 768 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | JAG2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene JAG2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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