CRISPR-U™ Gene Knockout Cell Line Strategy

JAG2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human JAG2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol JAG2
Gene id 3714
Organism Homo sapiens
Official full symbol jagged canonical Notch ligand 2
Gene type protein-coding
Also known as HJ2, SER2
Summary The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 14
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
JAG2-201 ENST00000331782.8 5773 1238aa Protein coding CCDS9998 Q9Y219-1 NM_002226.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
JAG2-202 ENST00000347004.2 5720 1200aa Protein coding CCDS9999 Q9Y219-2 - TSL:1, GENCODE basic,
JAG2-203 ENST00000546616.1 2275 No protein Retained intron - - - TSL:5,
JAG2-205 ENST00000553244.1 866 No protein Retained intron - - - TSL:3,
JAG2-204 ENST00000553051.1 768 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene JAG2
This KO Strategy loading
Red Cotton™ Notes Gene JAG2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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