CRISPR-U™ Gene Knockout Cell Line
Strategy
ACADVL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACADVL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ACADVL |
| Gene id | 37 |
| Organism | Homo sapiens |
| Official full symbol | acyl-CoA dehydrogenase very long chain |
| Gene type | protein-coding |
| Also known as | ACAD6, LCACD, VLCAD |
| Summary | The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ACADVL-205 | ENST00000543245.6 | 2227 | 678aa | Protein coding | CCDS58509 | P49748-3 | - | TSL:2, GENCODE basic, |
| ACADVL-202 | ENST00000350303.9 | 2191 | 633aa | Protein coding | CCDS42249 | P49748-2 | - | TSL:1, GENCODE basic, |
| ACADVL-203 | ENST00000356839.10 | 2184 | 655aa | Protein coding | CCDS11090 | P49748-1 | NM_000018.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ACADVL-204 | ENST00000542255.6 | 1016 | 282aa | Protein coding | - | G3V1M7 | - | CDS 5' incomplete, TSL:5, |
| ACADVL-233 | ENST00000583312.5 | 799 | 224aa | Protein coding | - | K7EQP4 | - | CDS 3' incomplete, TSL:3, |
| ACADVL-220 | ENST00000579546.1 | 582 | 194aa | Protein coding | - | J3QRJ8 | - | CDS 5' and 3' incomplete, TSL:5, |
| ACADVL-238 | ENST00000584103.5 | 555 | 157aa | Protein coding | - | K7EJW8 | - | CDS 3' incomplete, TSL:4, |
| ACADVL-221 | ENST00000579886.2 | 502 | 155aa | Protein coding | - | J3QKU9 | - | CDS 3' incomplete, TSL:5, |
| ACADVL-201 | ENST00000322910.9 | 2352 | 39aa | Nonsense mediated decay | - | J9JID6 | - | TSL:2, |
| ACADVL-237 | ENST00000583858.5 | 1015 | 204aa | Nonsense mediated decay | - | J3KSR4 | - | CDS 5' incomplete, TSL:5, |
| ACADVL-225 | ENST00000581378.5 | 752 | 76aa | Nonsense mediated decay | - | J3KS89 | - | CDS 5' incomplete, TSL:3, |
| ACADVL-235 | ENST00000583848.5 | 503 | 45aa | Nonsense mediated decay | - | K7EMF8 | - | CDS 5' incomplete, TSL:3, |
| ACADVL-226 | ENST00000581562.5 | 571 | No protein | Processed transcript | - | - | - | TSL:3, |
| ACADVL-208 | ENST00000577857.5 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| ACADVL-232 | ENST00000583074.5 | 410 | No protein | Processed transcript | - | - | - | TSL:5, |
| ACADVL-214 | ENST00000578711.1 | 1732 | No protein | Retained intron | - | - | - | TSL:NA, |
| ACADVL-219 | ENST00000579425.5 | 1234 | No protein | Retained intron | - | - | - | TSL:5, |
| ACADVL-206 | ENST00000577191.5 | 1212 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-239 | ENST00000585203.6 | 947 | No protein | Retained intron | - | - | - | TSL:5, |
| ACADVL-217 | ENST00000579286.5 | 933 | No protein | Retained intron | - | - | - | TSL:3, |
| ACADVL-236 | ENST00000583850.5 | 903 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-230 | ENST00000582379.1 | 833 | No protein | Retained intron | - | - | - | TSL:3, |
| ACADVL-207 | ENST00000577433.5 | 803 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-227 | ENST00000582056.5 | 738 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-210 | ENST00000578269.5 | 724 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-216 | ENST00000578824.5 | 720 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-211 | ENST00000578319.5 | 718 | No protein | Retained intron | - | - | - | TSL:3, |
| ACADVL-215 | ENST00000578809.5 | 709 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-229 | ENST00000582356.5 | 633 | No protein | Retained intron | - | - | - | TSL:4, |
| ACADVL-234 | ENST00000583760.1 | 599 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-209 | ENST00000578033.1 | 562 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-228 | ENST00000582166.1 | 550 | No protein | Retained intron | - | - | - | TSL:4, |
| ACADVL-212 | ENST00000578421.1 | 548 | No protein | Retained intron | - | - | - | TSL:3, |
| ACADVL-223 | ENST00000580263.5 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-224 | ENST00000580365.1 | 494 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-213 | ENST00000578579.2 | 458 | No protein | Retained intron | - | - | - | TSL:3, |
| ACADVL-222 | ENST00000579894.5 | 429 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-218 | ENST00000579391.1 | 341 | No protein | Retained intron | - | - | - | TSL:2, |
| ACADVL-231 | ENST00000582450.1 | 294 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ACADVL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ACADVL had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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