CRISPR-U™ Gene Knockout Cell Line Strategy

ACADVL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACADVL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ACADVL
Gene id 37
Organism Homo sapiens
Official full symbol acyl-CoA dehydrogenase very long chain
Gene type protein-coding
Also known as ACAD6, LCACD, VLCAD
Summary The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ACADVL-205 ENST00000543245.6 2227 678aa Protein coding CCDS58509 P49748-3 - TSL:2, GENCODE basic,
ACADVL-202 ENST00000350303.9 2191 633aa Protein coding CCDS42249 P49748-2 - TSL:1, GENCODE basic,
ACADVL-203 ENST00000356839.10 2184 655aa Protein coding CCDS11090 P49748-1 NM_000018.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ACADVL-204 ENST00000542255.6 1016 282aa Protein coding - G3V1M7 - CDS 5' incomplete, TSL:5,
ACADVL-233 ENST00000583312.5 799 224aa Protein coding - K7EQP4 - CDS 3' incomplete, TSL:3,
ACADVL-220 ENST00000579546.1 582 194aa Protein coding - J3QRJ8 - CDS 5' and 3' incomplete, TSL:5,
ACADVL-238 ENST00000584103.5 555 157aa Protein coding - K7EJW8 - CDS 3' incomplete, TSL:4,
ACADVL-221 ENST00000579886.2 502 155aa Protein coding - J3QKU9 - CDS 3' incomplete, TSL:5,
ACADVL-201 ENST00000322910.9 2352 39aa Nonsense mediated decay - J9JID6 - TSL:2,
ACADVL-237 ENST00000583858.5 1015 204aa Nonsense mediated decay - J3KSR4 - CDS 5' incomplete, TSL:5,
ACADVL-225 ENST00000581378.5 752 76aa Nonsense mediated decay - J3KS89 - CDS 5' incomplete, TSL:3,
ACADVL-235 ENST00000583848.5 503 45aa Nonsense mediated decay - K7EMF8 - CDS 5' incomplete, TSL:3,
ACADVL-226 ENST00000581562.5 571 No protein Processed transcript - - - TSL:3,
ACADVL-208 ENST00000577857.5 566 No protein Processed transcript - - - TSL:4,
ACADVL-232 ENST00000583074.5 410 No protein Processed transcript - - - TSL:5,
ACADVL-214 ENST00000578711.1 1732 No protein Retained intron - - - TSL:NA,
ACADVL-219 ENST00000579425.5 1234 No protein Retained intron - - - TSL:5,
ACADVL-206 ENST00000577191.5 1212 No protein Retained intron - - - TSL:2,
ACADVL-239 ENST00000585203.6 947 No protein Retained intron - - - TSL:5,
ACADVL-217 ENST00000579286.5 933 No protein Retained intron - - - TSL:3,
ACADVL-236 ENST00000583850.5 903 No protein Retained intron - - - TSL:2,
ACADVL-230 ENST00000582379.1 833 No protein Retained intron - - - TSL:3,
ACADVL-207 ENST00000577433.5 803 No protein Retained intron - - - TSL:2,
ACADVL-227 ENST00000582056.5 738 No protein Retained intron - - - TSL:2,
ACADVL-210 ENST00000578269.5 724 No protein Retained intron - - - TSL:2,
ACADVL-216 ENST00000578824.5 720 No protein Retained intron - - - TSL:2,
ACADVL-211 ENST00000578319.5 718 No protein Retained intron - - - TSL:3,
ACADVL-215 ENST00000578809.5 709 No protein Retained intron - - - TSL:2,
ACADVL-229 ENST00000582356.5 633 No protein Retained intron - - - TSL:4,
ACADVL-234 ENST00000583760.1 599 No protein Retained intron - - - TSL:2,
ACADVL-209 ENST00000578033.1 562 No protein Retained intron - - - TSL:2,
ACADVL-228 ENST00000582166.1 550 No protein Retained intron - - - TSL:4,
ACADVL-212 ENST00000578421.1 548 No protein Retained intron - - - TSL:3,
ACADVL-223 ENST00000580263.5 545 No protein Retained intron - - - TSL:2,
ACADVL-224 ENST00000580365.1 494 No protein Retained intron - - - TSL:2,
ACADVL-213 ENST00000578579.2 458 No protein Retained intron - - - TSL:3,
ACADVL-222 ENST00000579894.5 429 No protein Retained intron - - - TSL:2,
ACADVL-218 ENST00000579391.1 341 No protein Retained intron - - - TSL:2,
ACADVL-231 ENST00000582450.1 294 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ACADVL
This KO Strategy loading
Red Cotton™ Notes Gene ACADVL had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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