CRISPR-U™ Gene Knockout Cell Line Strategy

IRF7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IRF7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IRF7
Gene id 3665
Organism Homo sapiens
Official full symbol interferon regulatory factor 7
Gene type protein-coding
Also known as IMD39, IRF-7, IRF-7H, IRF7A, IRF7B, IRF7C, IRF7H
Summary IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
Genomic regions Chromosome 11
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IRF7-203 ENST00000397566.5 2051 516aa Protein coding CCDS7705 Q92985-4 - TSL:1, GENCODE basic, APPRIS P4,
IRF7-201 ENST00000330243.9 1992 516aa Protein coding CCDS7705 Q92985-4 - TSL:5, GENCODE basic, APPRIS P4,
IRF7-206 ENST00000525445.6 1923 503aa Protein coding CCDS7703 Q92985-1 NM_001572.5 TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
IRF7-202 ENST00000348655.11 1807 474aa Protein coding CCDS7704 Q92985-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IRF7-204 ENST00000397570.5 1943 487aa Protein coding - M9RSF4 - TSL:1, GENCODE basic, APPRIS ALT2,
IRF7-216 ENST00000647801.1 891 161aa Protein coding - A0A3B3ISS4 - CDS 3' incomplete,
IRF7-217 ENST00000649187.1 745 154aa Protein coding - A0A3B3ISC0 - CDS 3' incomplete,
IRF7-214 ENST00000533182.5 1776 164aa Nonsense mediated decay - Q92985-3 - TSL:2,
IRF7-212 ENST00000532326.5 1604 185aa Nonsense mediated decay - E9PIA7 - TSL:5,
IRF7-205 ENST00000469048.6 1596 164aa Nonsense mediated decay - Q92985-3 - TSL:1,
IRF7-209 ENST00000528413.6 1554 55aa Nonsense mediated decay - A0A3B3ITQ5 - CDS 5' incomplete, TSL:5,
IRF7-213 ENST00000532788.1 1019 177aa Nonsense mediated decay - E9PR03 - TSL:5,
IRF7-215 ENST00000533190.1 806 135aa Nonsense mediated decay - E9PQ11 - TSL:2,
IRF7-211 ENST00000532096.1 1049 No protein Retained intron - - - TSL:2,
IRF7-210 ENST00000531912.1 806 No protein Retained intron - - - TSL:2,
IRF7-207 ENST00000525750.1 656 No protein Retained intron - - - TSL:1,
IRF7-208 ENST00000527160.1 617 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IRF7
This KO Strategy loading
Red Cotton™ Notes Gene IRF7 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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