CRISPR-U™ Gene Knockout Cell Line
Strategy
IRF7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IRF7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IRF7 |
| Gene id | 3665 |
| Organism | Homo sapiens |
| Official full symbol | interferon regulatory factor 7 |
| Gene type | protein-coding |
| Also known as | IMD39, IRF-7, IRF-7H, IRF7A, IRF7B, IRF7C, IRF7H |
| Summary | IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IRF7-203 | ENST00000397566.5 | 2051 | 516aa | Protein coding | CCDS7705 | Q92985-4 | - | TSL:1, GENCODE basic, APPRIS P4, |
| IRF7-201 | ENST00000330243.9 | 1992 | 516aa | Protein coding | CCDS7705 | Q92985-4 | - | TSL:5, GENCODE basic, APPRIS P4, |
| IRF7-206 | ENST00000525445.6 | 1923 | 503aa | Protein coding | CCDS7703 | Q92985-1 | NM_001572.5 | TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| IRF7-202 | ENST00000348655.11 | 1807 | 474aa | Protein coding | CCDS7704 | Q92985-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IRF7-204 | ENST00000397570.5 | 1943 | 487aa | Protein coding | - | M9RSF4 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IRF7-216 | ENST00000647801.1 | 891 | 161aa | Protein coding | - | A0A3B3ISS4 | - | CDS 3' incomplete, |
| IRF7-217 | ENST00000649187.1 | 745 | 154aa | Protein coding | - | A0A3B3ISC0 | - | CDS 3' incomplete, |
| IRF7-214 | ENST00000533182.5 | 1776 | 164aa | Nonsense mediated decay | - | Q92985-3 | - | TSL:2, |
| IRF7-212 | ENST00000532326.5 | 1604 | 185aa | Nonsense mediated decay | - | E9PIA7 | - | TSL:5, |
| IRF7-205 | ENST00000469048.6 | 1596 | 164aa | Nonsense mediated decay | - | Q92985-3 | - | TSL:1, |
| IRF7-209 | ENST00000528413.6 | 1554 | 55aa | Nonsense mediated decay | - | A0A3B3ITQ5 | - | CDS 5' incomplete, TSL:5, |
| IRF7-213 | ENST00000532788.1 | 1019 | 177aa | Nonsense mediated decay | - | E9PR03 | - | TSL:5, |
| IRF7-215 | ENST00000533190.1 | 806 | 135aa | Nonsense mediated decay | - | E9PQ11 | - | TSL:2, |
| IRF7-211 | ENST00000532096.1 | 1049 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF7-210 | ENST00000531912.1 | 806 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF7-207 | ENST00000525750.1 | 656 | No protein | Retained intron | - | - | - | TSL:1, |
| IRF7-208 | ENST00000527160.1 | 617 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IRF7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IRF7 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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