CRISPR-U™ Gene Knockout Cell Line
Strategy
IRF3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IRF3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IRF3 |
| Gene id | 3661 |
| Organism | Homo sapiens |
| Official full symbol | interferon regulatory factor 3 |
| Gene type | protein-coding |
| Also known as | IIAE7 |
| Summary | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important in the innate immune response against DNA and RNA viruses. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 22 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IRF3-217 | ENST00000597198.5 | 1741 | 427aa | Protein coding | CCDS12775 | Q14653-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| IRF3-201 | ENST00000309877.11 | 1691 | 427aa | Protein coding | CCDS12775 | Q14653-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| IRF3-203 | ENST00000377139.8 | 1595 | 427aa | Protein coding | CCDS12775 | Q14653-1 | NM_001571.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IRF3-228 | ENST00000601291.5 | 1556 | 452aa | Protein coding | CCDS59409 | Q14653-4 | - | TSL:1, GENCODE basic, |
| IRF3-207 | ENST00000593922.5 | 1494 | 281aa | Protein coding | CCDS59408 | M0QYT9 | - | TSL:1, GENCODE basic, |
| IRF3-221 | ENST00000598808.5 | 1468 | 281aa | Protein coding | CCDS59408 | M0QYT9 | - | TSL:5, GENCODE basic, |
| IRF3-202 | ENST00000377135.8 | 1442 | 281aa | Protein coding | CCDS59408 | M0QYT9 | - | TSL:1, GENCODE basic, |
| IRF3-222 | ENST00000599144.5 | 1117 | 281aa | Protein coding | CCDS59408 | M0QYT9 | - | TSL:1, GENCODE basic, |
| IRF3-223 | ENST00000599223.5 | 1055 | 300aa | Protein coding | CCDS56099 | Q14653-2 | - | TSL:1, GENCODE basic, |
| IRF3-225 | ENST00000600022.5 | 882 | 154aa | Protein coding | CCDS59407 | Q14653-3 | - | TSL:1, GENCODE basic, |
| IRF3-213 | ENST00000596765.5 | 710 | 154aa | Protein coding | CCDS59407 | Q14653-3 | - | TSL:1, GENCODE basic, |
| IRF3-227 | ENST00000600911.5 | 1637 | 408aa | Protein coding | - | M0QZB7 | - | TSL:5, GENCODE basic, |
| IRF3-220 | ENST00000598108.5 | 1118 | 287aa | Protein coding | - | M0R205 | - | CDS 3' incomplete, TSL:3, |
| IRF3-212 | ENST00000596756.5 | 931 | 264aa | Protein coding | - | M0R3C2 | - | CDS 3' incomplete, TSL:5, |
| IRF3-206 | ENST00000593818.5 | 924 | 105aa | Protein coding | - | M0R0X9 | - | CDS 3' incomplete, TSL:2, |
| IRF3-215 | ENST00000596822.5 | 823 | 135aa | Protein coding | - | M0QX56 | - | TSL:3, GENCODE basic, |
| IRF3-230 | ENST00000601809.5 | 768 | 190aa | Protein coding | - | M0QYX3 | - | CDS 3' incomplete, TSL:2, |
| IRF3-209 | ENST00000595034.5 | 739 | 119aa | Protein coding | - | M0QXC8 | - | CDS 3' incomplete, TSL:3, |
| IRF3-226 | ENST00000600453.1 | 700 | 102aa | Protein coding | - | M0R007 | - | CDS 3' incomplete, TSL:3, |
| IRF3-229 | ENST00000601373.5 | 680 | 84aa | Protein coding | - | M0R0R8 | - | CDS 3' incomplete, TSL:2, |
| IRF3-205 | ENST00000593337.5 | 633 | 153aa | Protein coding | - | M0R3E9 | - | CDS 3' incomplete, TSL:3, |
| IRF3-204 | ENST00000442265.2 | 315 | 104aa | Protein coding | - | Q14653-5 | - | TSL:1, GENCODE basic, |
| IRF3-219 | ENST00000597636.5 | 2874 | 329aa | Nonsense mediated decay | - | M0QZB8 | - | TSL:5, |
| IRF3-214 | ENST00000596788.1 | 447 | 71aa | Nonsense mediated decay | - | M0R3H7 | - | CDS 5' incomplete, TSL:3, |
| IRF3-224 | ENST00000599680.1 | 472 | No protein | Processed transcript | - | - | - | TSL:3, |
| IRF3-231 | ENST00000602190.1 | 413 | No protein | Processed transcript | - | - | - | TSL:3, |
| IRF3-216 | ENST00000597180.1 | 301 | No protein | Processed transcript | - | - | - | TSL:5, |
| IRF3-208 | ENST00000594387.1 | 800 | No protein | Retained intron | - | - | - | TSL:3, |
| IRF3-210 | ENST00000595240.5 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF3-211 | ENST00000596644.5 | 550 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF3-218 | ENST00000597369.1 | 550 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IRF3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IRF3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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