CRISPR-U™ Gene Knockout Cell Line Strategy

IRF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IRF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IRF2
Gene id 3660
Organism Homo sapiens
Official full symbol interferon regulatory factor 2
Gene type protein-coding
Also known as IRF-2
Summary IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.
Genomic regions Chromosome 4
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IRF2-201 ENST00000393593.8 2257 349aa Protein coding CCDS3835 P14316-1 NM_002199.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IRF2-206 ENST00000507523.5 595 176aa Protein coding - K4DIA5 - CDS 3' incomplete, TSL:4,
IRF2-204 ENST00000505067.5 586 196aa Protein coding - K4DIA4 - CDS 5' and 3' incomplete, TSL:3,
IRF2-208 ENST00000510814.5 568 160aa Protein coding - D6RB08 - CDS 3' incomplete, TSL:4,
IRF2-205 ENST00000506230.5 498 137aa Protein coding - D6R9N5 - CDS 3' incomplete, TSL:2,
IRF2-202 ENST00000502750.1 339 113aa Protein coding - H0Y956 - CDS 5' and 3' incomplete, TSL:3,
IRF2-207 ENST00000509274.1 591 83aa Nonsense mediated decay - D6RED1 - TSL:4,
IRF2-209 ENST00000512020.5 731 No protein Processed transcript - - - TSL:3,
IRF2-203 ENST00000504340.1 565 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IRF2
This KO Strategy loading
Red Cotton™ Notes Gene IRF2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×