CRISPR-U™ Gene Knockout Cell Line
Strategy
IRF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IRF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IRF2 |
| Gene id | 3660 |
| Organism | Homo sapiens |
| Official full symbol | interferon regulatory factor 2 |
| Gene type | protein-coding |
| Also known as | IRF-2 |
| Summary | IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IRF2-201 | ENST00000393593.8 | 2257 | 349aa | Protein coding | CCDS3835 | P14316-1 | NM_002199.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IRF2-206 | ENST00000507523.5 | 595 | 176aa | Protein coding | - | K4DIA5 | - | CDS 3' incomplete, TSL:4, |
| IRF2-204 | ENST00000505067.5 | 586 | 196aa | Protein coding | - | K4DIA4 | - | CDS 5' and 3' incomplete, TSL:3, |
| IRF2-208 | ENST00000510814.5 | 568 | 160aa | Protein coding | - | D6RB08 | - | CDS 3' incomplete, TSL:4, |
| IRF2-205 | ENST00000506230.5 | 498 | 137aa | Protein coding | - | D6R9N5 | - | CDS 3' incomplete, TSL:2, |
| IRF2-202 | ENST00000502750.1 | 339 | 113aa | Protein coding | - | H0Y956 | - | CDS 5' and 3' incomplete, TSL:3, |
| IRF2-207 | ENST00000509274.1 | 591 | 83aa | Nonsense mediated decay | - | D6RED1 | - | TSL:4, |
| IRF2-209 | ENST00000512020.5 | 731 | No protein | Processed transcript | - | - | - | TSL:3, |
| IRF2-203 | ENST00000504340.1 | 565 | No protein | Processed transcript | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IRF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IRF2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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