CRISPR-U™ Gene Knockout Cell Line
Strategy
ITGA6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ITGA6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ITGA6 |
| Gene id | 3655 |
| Organism | Homo sapiens |
| Official full symbol | integrin subunit alpha 6 |
| Gene type | protein-coding |
| Also known as | CD49f, ITGA6B, VLA-6 |
| Summary | The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ITGA6-202 | ENST00000409080.6 | 5686 | 1091aa | Protein coding | CCDS46451 | P23229-3 | NM_001079818.3 | TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| ITGA6-201 | ENST00000264107.11 | 5618 | 1073aa | Protein coding | CCDS2249 | P23229-2 | - | TSL:1, GENCODE basic, APPRIS P3, |
| ITGA6-203 | ENST00000409532.5 | 3544 | 954aa | Protein coding | CCDS82534 | P23229-7 | - | TSL:2, GENCODE basic, |
| ITGA6-206 | ENST00000442250.5 | 3393 | 1130aa | Protein coding | - | P23229-1 | - | TSL:5, GENCODE basic, |
| ITGA6-207 | ENST00000458358.5 | 3261 | 1086aa | Protein coding | - | P23229-5 | - | TSL:5, GENCODE basic, |
| ITGA6-204 | ENST00000412899.5 | 970 | 231aa | Protein coding | - | C9JXX7 | - | CDS 3' incomplete, TSL:1, |
| ITGA6-205 | ENST00000416789.1 | 853 | 258aa | Protein coding | - | H7BZ97 | - | CDS 5' incomplete, TSL:3, |
| ITGA6-208 | ENST00000469534.1 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
| ITGA6-210 | ENST00000475302.1 | 577 | No protein | Retained intron | - | - | - | TSL:3, |
| ITGA6-211 | ENST00000497107.1 | 573 | No protein | Retained intron | - | - | - | TSL:2, |
| ITGA6-209 | ENST00000470259.1 | 365 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ITGA6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ITGA6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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