CRISPR-U™ Gene Knockout Cell Line Strategy

ITGA6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ITGA6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ITGA6
Gene id 3655
Organism Homo sapiens
Official full symbol integrin subunit alpha 6
Gene type protein-coding
Also known as CD49f, ITGA6B, VLA-6
Summary The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 2
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ITGA6-202 ENST00000409080.6 5686 1091aa Protein coding CCDS46451 P23229-3 NM_001079818.3 TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
ITGA6-201 ENST00000264107.11 5618 1073aa Protein coding CCDS2249 P23229-2 - TSL:1, GENCODE basic, APPRIS P3,
ITGA6-203 ENST00000409532.5 3544 954aa Protein coding CCDS82534 P23229-7 - TSL:2, GENCODE basic,
ITGA6-206 ENST00000442250.5 3393 1130aa Protein coding - P23229-1 - TSL:5, GENCODE basic,
ITGA6-207 ENST00000458358.5 3261 1086aa Protein coding - P23229-5 - TSL:5, GENCODE basic,
ITGA6-204 ENST00000412899.5 970 231aa Protein coding - C9JXX7 - CDS 3' incomplete, TSL:1,
ITGA6-205 ENST00000416789.1 853 258aa Protein coding - H7BZ97 - CDS 5' incomplete, TSL:3,
ITGA6-208 ENST00000469534.1 581 No protein Retained intron - - - TSL:2,
ITGA6-210 ENST00000475302.1 577 No protein Retained intron - - - TSL:3,
ITGA6-211 ENST00000497107.1 573 No protein Retained intron - - - TSL:2,
ITGA6-209 ENST00000470259.1 365 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ITGA6
This KO Strategy loading
Red Cotton™ Notes Gene ITGA6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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