CRISPR-U™ Gene Knockout Cell Line
Strategy
IL15RA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL15RA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL15RA |
| Gene id | 3601 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 15 receptor subunit alpha |
| Gene type | protein-coding |
| Also known as | CD215 |
| Summary | This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 18 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL15RA-214 | ENST00000525219.6 | 1850 | 231aa | Protein coding | CCDS58069 | Q13261-10 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-206 | ENST00000397248.6 | 1799 | 353aa | Protein coding | CCDS73065 | A0A0A0MS77 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-204 | ENST00000379977.8 | 1566 | 267aa | Protein coding | CCDS7074 | Q13261-1 | NM_002189.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| IL15RA-215 | ENST00000528354.5 | 1037 | 234aa | Protein coding | CCDS7075 | Q13261-3 | - | TSL:2, GENCODE basic, |
| IL15RA-222 | ENST00000622442.4 | 1160 | 346aa | Protein coding | - | K9N2Q6 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-220 | ENST00000620345.4 | 1115 | 331aa | Protein coding | - | K9N163 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-208 | ENST00000397251.7 | 1046 | 308aa | Protein coding | - | K9N1J3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-221 | ENST00000620865.4 | 1037 | 305aa | Protein coding | - | K9N2S2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IL15RA-212 | ENST00000447291.5 | 906 | 155aa | Protein coding | - | H0Y6E8 | - | CDS 5' incomplete, TSL:3, |
| IL15RA-205 | ENST00000397246.7 | 858 | 172aa | Protein coding | - | E7ETI1 | - | CDS 3' incomplete, TSL:5, |
| IL15RA-209 | ENST00000397255.7 | 759 | 252aa | Protein coding | - | Q13261-4 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IL15RA-213 | ENST00000453922.1 | 730 | 148aa | Protein coding | - | Q5JVA3 | - | CDS 3' incomplete, TSL:3, |
| IL15RA-217 | ENST00000532039.5 | 679 | 209aa | Protein coding | - | H0YD11 | - | CDS 5' incomplete, TSL:5, |
| IL15RA-216 | ENST00000530685.5 | 660 | 219aa | Protein coding | - | Q13261-5 | - | TSL:5, GENCODE basic, |
| IL15RA-211 | ENST00000435171.6 | 659 | 198aa | Protein coding | - | H0Y4R3 | - | CDS 5' incomplete, TSL:2, |
| IL15RA-201 | ENST00000379971.5 | 588 | 154aa | Protein coding | - | Q13261-9 | - | TSL:3, GENCODE basic, |
| IL15RA-207 | ENST00000397250.6 | 584 | 169aa | Protein coding | - | Q13261-8 | - | TSL:2, GENCODE basic, |
| IL15RA-210 | ENST00000429135.2 | 547 | 172aa | Protein coding | - | E9PQ64 | - | CDS 3' incomplete, TSL:4, |
| IL15RA-219 | ENST00000534292.5 | 1694 | No protein | Processed transcript | - | - | - | TSL:1, |
| IL15RA-202 | ENST00000379972.6 | 1631 | No protein | Processed transcript | - | - | - | TSL:5, |
| IL15RA-218 | ENST00000532948.5 | 1581 | No protein | Processed transcript | - | - | - | TSL:3, |
| IL15RA-203 | ENST00000379974.1 | 1022 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL15RA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL15RA had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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