CRISPR-U™ Gene Knockout Cell Line Strategy

IL15RA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IL15RA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IL15RA
Gene id 3601
Organism Homo sapiens
Official full symbol interleukin 15 receptor subunit alpha
Gene type protein-coding
Also known as CD215
Summary This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
Genomic regions Chromosome 10
Strategy Summary
This gene has 18 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IL15RA-214 ENST00000525219.6 1850 231aa Protein coding CCDS58069 Q13261-10 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-206 ENST00000397248.6 1799 353aa Protein coding CCDS73065 A0A0A0MS77 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-204 ENST00000379977.8 1566 267aa Protein coding CCDS7074 Q13261-1 NM_002189.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
IL15RA-215 ENST00000528354.5 1037 234aa Protein coding CCDS7075 Q13261-3 - TSL:2, GENCODE basic,
IL15RA-222 ENST00000622442.4 1160 346aa Protein coding - K9N2Q6 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-220 ENST00000620345.4 1115 331aa Protein coding - K9N163 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-208 ENST00000397251.7 1046 308aa Protein coding - K9N1J3 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-221 ENST00000620865.4 1037 305aa Protein coding - K9N2S2 - TSL:1, GENCODE basic, APPRIS ALT2,
IL15RA-212 ENST00000447291.5 906 155aa Protein coding - H0Y6E8 - CDS 5' incomplete, TSL:3,
IL15RA-205 ENST00000397246.7 858 172aa Protein coding - E7ETI1 - CDS 3' incomplete, TSL:5,
IL15RA-209 ENST00000397255.7 759 252aa Protein coding - Q13261-4 - TSL:5, GENCODE basic, APPRIS ALT2,
IL15RA-213 ENST00000453922.1 730 148aa Protein coding - Q5JVA3 - CDS 3' incomplete, TSL:3,
IL15RA-217 ENST00000532039.5 679 209aa Protein coding - H0YD11 - CDS 5' incomplete, TSL:5,
IL15RA-216 ENST00000530685.5 660 219aa Protein coding - Q13261-5 - TSL:5, GENCODE basic,
IL15RA-211 ENST00000435171.6 659 198aa Protein coding - H0Y4R3 - CDS 5' incomplete, TSL:2,
IL15RA-201 ENST00000379971.5 588 154aa Protein coding - Q13261-9 - TSL:3, GENCODE basic,
IL15RA-207 ENST00000397250.6 584 169aa Protein coding - Q13261-8 - TSL:2, GENCODE basic,
IL15RA-210 ENST00000429135.2 547 172aa Protein coding - E9PQ64 - CDS 3' incomplete, TSL:4,
IL15RA-219 ENST00000534292.5 1694 No protein Processed transcript - - - TSL:1,
IL15RA-202 ENST00000379972.6 1631 No protein Processed transcript - - - TSL:5,
IL15RA-218 ENST00000532948.5 1581 No protein Processed transcript - - - TSL:3,
IL15RA-203 ENST00000379974.1 1022 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IL15RA
This KO Strategy loading
Red Cotton™ Notes Gene IL15RA had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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