CRISPR-U™ Gene Knockout Cell Line Strategy

IL7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IL7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IL7
Gene id 3574
Organism Homo sapiens
Official full symbol interleukin 7
Gene type protein-coding
Also known as IL-7
Summary The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19).
Genomic regions Chromosome 8
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IL7-210 ENST00000541183.2 2020 159aa Protein coding CCDS75756 A0A0A0MTG5 - TSL:5, GENCODE basic,
IL7-201 ENST00000263851.9 2016 177aa Protein coding CCDS6224 P13232-1 NM_000880.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IL7-202 ENST00000379113.6 1888 115aa Protein coding CCDS75755 Q5FBX5 - TSL:5, GENCODE basic,
IL7-206 ENST00000520269.5 456 133aa Protein coding CCDS56541 P13232-2 - TSL:3, GENCODE basic,
IL7-211 ENST00000639719.1 597 71aa Protein coding - Q5FBY3 - TSL:5, GENCODE basic,
IL7-203 ENST00000518982.5 1940 71aa Nonsense mediated decay - Q5FBY3 - TSL:1,
IL7-207 ENST00000520317.1 1055 37aa Nonsense mediated decay - Q5FBY6 - TSL:1,
IL7-205 ENST00000520215.5 400 37aa Nonsense mediated decay - Q5FBY6 - TSL:5,
IL7-204 ENST00000519833.5 721 No protein Processed transcript - - - TSL:5,
IL7-209 ENST00000523959.5 635 No protein Processed transcript - - - TSL:3,
IL7-208 ENST00000521323.1 583 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IL7
This KO Strategy loading
Red Cotton™ Notes Gene IL7 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×