CRISPR-U™ Gene Knockout Cell Line
Strategy
IL7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL7 |
| Gene id | 3574 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 7 |
| Gene type | protein-coding |
| Also known as | IL-7 |
| Summary | The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19). |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL7-210 | ENST00000541183.2 | 2020 | 159aa | Protein coding | CCDS75756 | A0A0A0MTG5 | - | TSL:5, GENCODE basic, |
| IL7-201 | ENST00000263851.9 | 2016 | 177aa | Protein coding | CCDS6224 | P13232-1 | NM_000880.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IL7-202 | ENST00000379113.6 | 1888 | 115aa | Protein coding | CCDS75755 | Q5FBX5 | - | TSL:5, GENCODE basic, |
| IL7-206 | ENST00000520269.5 | 456 | 133aa | Protein coding | CCDS56541 | P13232-2 | - | TSL:3, GENCODE basic, |
| IL7-211 | ENST00000639719.1 | 597 | 71aa | Protein coding | - | Q5FBY3 | - | TSL:5, GENCODE basic, |
| IL7-203 | ENST00000518982.5 | 1940 | 71aa | Nonsense mediated decay | - | Q5FBY3 | - | TSL:1, |
| IL7-207 | ENST00000520317.1 | 1055 | 37aa | Nonsense mediated decay | - | Q5FBY6 | - | TSL:1, |
| IL7-205 | ENST00000520215.5 | 400 | 37aa | Nonsense mediated decay | - | Q5FBY6 | - | TSL:5, |
| IL7-204 | ENST00000519833.5 | 721 | No protein | Processed transcript | - | - | - | TSL:5, |
| IL7-209 | ENST00000523959.5 | 635 | No protein | Processed transcript | - | - | - | TSL:3, |
| IL7-208 | ENST00000521323.1 | 583 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL7 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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