CRISPR-U™ Gene Knockout Cell Line Strategy

IL4R Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IL4R Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IL4R
Gene id 3566
Organism Homo sapiens
Official full symbol interleukin 4 receptor
Gene type protein-coding
Also known as CD124, IL-4RA, IL4RA
Summary This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 16
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IL4R-202 ENST00000395762.7 3624 825aa Protein coding CCDS10629 P24394-1 NM_000418.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
IL4R-203 ENST00000543915.6 3539 825aa Protein coding CCDS10629 P24394-1 - TSL:1, GENCODE basic, APPRIS P2,
IL4R-201 ENST00000170630.6 3380 810aa Protein coding CCDS58441 P24394-3 - TSL:5, GENCODE basic, APPRIS ALT2,
IL4R-212 ENST00000565179.5 579 193aa Protein coding - I3L4W9 - CDS 5' and 3' incomplete, TSL:3,
IL4R-208 ENST00000563002.5 541 120aa Protein coding - J9JII2 - CDS 3' incomplete, TSL:5,
IL4R-207 ENST00000562968.1 531 49aa Protein coding - H3BSE7 - CDS 5' incomplete, TSL:3,
IL4R-216 ENST00000566117.5 529 120aa Protein coding - J9JII2 - CDS 3' incomplete, TSL:5,
IL4R-206 ENST00000562142.5 518 63aa Protein coding - H3BTD9 - CDS 3' incomplete, TSL:3,
IL4R-213 ENST00000565352.1 424 133aa Protein coding - I3L4H7 - CDS 5' incomplete, TSL:5,
IL4R-204 ENST00000561742.5 379 10aa Protein coding - A0A0G2JLA1 - CDS 3' incomplete, TSL:3,
IL4R-219 ENST00000628578.1 201 66aa Protein coding - Q5FC08 - TSL:1, GENCODE basic,
IL4R-218 ENST00000568746.5 2900 66aa Nonsense mediated decay - Q5FC08 - TSL:2,
IL4R-217 ENST00000566318.5 688 124aa Nonsense mediated decay - H3BRH7 - TSL:3,
IL4R-211 ENST00000563926.5 594 66aa Nonsense mediated decay - Q5FC08 - TSL:4,
IL4R-215 ENST00000565915.5 541 No protein Processed transcript - - - TSL:4,
IL4R-210 ENST00000563886.1 734 No protein Retained intron - - - TSL:2,
IL4R-209 ENST00000563787.1 701 No protein Retained intron - - - TSL:3,
IL4R-205 ENST00000561946.5 611 No protein Retained intron - - - TSL:3,
IL4R-214 ENST00000565696.1 533 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IL4R
This KO Strategy loading
Red Cotton™ Notes Gene IL4R had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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