CRISPR-U™ Gene Knockout Cell Line
Strategy
IL4R Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL4R Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL4R |
| Gene id | 3566 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 4 receptor |
| Gene type | protein-coding |
| Also known as | CD124, IL-4RA, IL4RA |
| Summary | This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL4R-202 | ENST00000395762.7 | 3624 | 825aa | Protein coding | CCDS10629 | P24394-1 | NM_000418.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| IL4R-203 | ENST00000543915.6 | 3539 | 825aa | Protein coding | CCDS10629 | P24394-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| IL4R-201 | ENST00000170630.6 | 3380 | 810aa | Protein coding | CCDS58441 | P24394-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IL4R-212 | ENST00000565179.5 | 579 | 193aa | Protein coding | - | I3L4W9 | - | CDS 5' and 3' incomplete, TSL:3, |
| IL4R-208 | ENST00000563002.5 | 541 | 120aa | Protein coding | - | J9JII2 | - | CDS 3' incomplete, TSL:5, |
| IL4R-207 | ENST00000562968.1 | 531 | 49aa | Protein coding | - | H3BSE7 | - | CDS 5' incomplete, TSL:3, |
| IL4R-216 | ENST00000566117.5 | 529 | 120aa | Protein coding | - | J9JII2 | - | CDS 3' incomplete, TSL:5, |
| IL4R-206 | ENST00000562142.5 | 518 | 63aa | Protein coding | - | H3BTD9 | - | CDS 3' incomplete, TSL:3, |
| IL4R-213 | ENST00000565352.1 | 424 | 133aa | Protein coding | - | I3L4H7 | - | CDS 5' incomplete, TSL:5, |
| IL4R-204 | ENST00000561742.5 | 379 | 10aa | Protein coding | - | A0A0G2JLA1 | - | CDS 3' incomplete, TSL:3, |
| IL4R-219 | ENST00000628578.1 | 201 | 66aa | Protein coding | - | Q5FC08 | - | TSL:1, GENCODE basic, |
| IL4R-218 | ENST00000568746.5 | 2900 | 66aa | Nonsense mediated decay | - | Q5FC08 | - | TSL:2, |
| IL4R-217 | ENST00000566318.5 | 688 | 124aa | Nonsense mediated decay | - | H3BRH7 | - | TSL:3, |
| IL4R-211 | ENST00000563926.5 | 594 | 66aa | Nonsense mediated decay | - | Q5FC08 | - | TSL:4, |
| IL4R-215 | ENST00000565915.5 | 541 | No protein | Processed transcript | - | - | - | TSL:4, |
| IL4R-210 | ENST00000563886.1 | 734 | No protein | Retained intron | - | - | - | TSL:2, |
| IL4R-209 | ENST00000563787.1 | 701 | No protein | Retained intron | - | - | - | TSL:3, |
| IL4R-205 | ENST00000561946.5 | 611 | No protein | Retained intron | - | - | - | TSL:3, |
| IL4R-214 | ENST00000565696.1 | 533 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL4R |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL4R had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: