CRISPR-U™ Gene Knockout Cell Line Strategy

FAS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FAS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FAS
Gene id 355
Organism Homo sapiens
Official full symbol Fas cell surface death receptor
Gene type protein-coding
Also known as ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6
Summary The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Genomic regions Chromosome 10
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FAS-221 ENST00000652046.1 3696 335aa Protein coding CCDS7393 P25445-1 NM_000043.6 GENCODE basic, APPRIS P4, MANE Select v0.92,
FAS-205 ENST00000357339.6 1044 314aa Protein coding CCDS7394 P25445-6 - TSL:1, GENCODE basic, APPRIS ALT2,
FAS-203 ENST00000355279.2 983 220aa Protein coding CCDS7395 P25445-7 - TSL:1, GENCODE basic,
FAS-204 ENST00000355740.7 2605 197aa Protein coding - K9J972 - TSL:1, GENCODE basic,
FAS-202 ENST00000352159.8 1722 233aa Protein coding - H7C5Z8 - TSL:5, GENCODE basic,
FAS-217 ENST00000612663.5 1464 244aa Nonsense mediated decay - A0A087WTM9 - TSL:5,
FAS-211 ENST00000484444.5 1076 103aa Nonsense mediated decay - P25445-2 - TSL:1,
FAS-215 ENST00000494410.5 975 149aa Nonsense mediated decay - P25445-4 - TSL:1,
FAS-213 ENST00000488877.5 924 149aa Nonsense mediated decay - P25445-4 - TSL:1,
FAS-210 ENST00000479522.5 857 86aa Nonsense mediated decay - P25445-3 - TSL:1,
FAS-214 ENST00000492756.5 836 132aa Nonsense mediated decay - P25445-5 - TSL:1,
FAS-218 ENST00000640140.1 2262 No protein Processed transcript - - - TSL:5,
FAS-220 ENST00000640681.1 2221 No protein Processed transcript - - - TSL:5,
FAS-219 ENST00000640250.1 1598 No protein Processed transcript - - - TSL:5,
FAS-201 ENST00000313771.9 1044 No protein Processed transcript - - - TSL:1,
FAS-212 ENST00000487314.1 824 No protein Processed transcript - - - TSL:5,
FAS-207 ENST00000460510.5 810 No protein Processed transcript - - - TSL:3,
FAS-209 ENST00000477270.5 731 No protein Processed transcript - - - TSL:5,
FAS-216 ENST00000494799.5 681 No protein Processed transcript - - - TSL:3,
FAS-208 ENST00000466081.5 625 No protein Processed transcript - - - TSL:3,
FAS-206 ENST00000371857.7 1023 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FAS
This KO Strategy loading
Red Cotton™ Notes Gene FAS had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

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