CRISPR-U™ Gene Knockout Cell Line Strategy

APOE Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human APOE Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol APOE
Gene id 348
Organism Homo sapiens
Official full symbol apolipoprotein E
Gene type protein-coding
Also known as AD2, APO-E, ApoE4, LDLCQ5, LPG
Summary The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
APOE-201 ENST00000252486.9 1166 317aa Protein coding CCDS12647 A0A0S2Z3D5 P02649 NM_000041.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
APOE-202 ENST00000425718.1 923 219aa Protein coding - E7ERP7 - CDS 3' incomplete, TSL:1,
APOE-203 ENST00000434152.5 864 269aa Protein coding - H0Y7L5 - CDS 3' incomplete, TSL:2,
APOE-204 ENST00000446996.5 737 216aa Protein coding - E9PEV4 - CDS 3' incomplete, TSL:2,
APOE-205 ENST00000485628.2 771 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene APOE
This KO Strategy loading
Red Cotton™ Notes Gene APOE had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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