CRISPR-U™ Gene Knockout Cell Line
Strategy
APOE Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human APOE Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | APOE |
| Gene id | 348 |
| Organism | Homo sapiens |
| Official full symbol | apolipoprotein E |
| Gene type | protein-coding |
| Also known as | AD2, APO-E, ApoE4, LDLCQ5, LPG |
| Summary | The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| APOE-201 | ENST00000252486.9 | 1166 | 317aa | Protein coding | CCDS12647 | A0A0S2Z3D5 P02649 | NM_000041.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| APOE-202 | ENST00000425718.1 | 923 | 219aa | Protein coding | - | E7ERP7 | - | CDS 3' incomplete, TSL:1, |
| APOE-203 | ENST00000434152.5 | 864 | 269aa | Protein coding | - | H0Y7L5 | - | CDS 3' incomplete, TSL:2, |
| APOE-204 | ENST00000446996.5 | 737 | 216aa | Protein coding | - | E9PEV4 | - | CDS 3' incomplete, TSL:2, |
| APOE-205 | ENST00000485628.2 | 771 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | APOE |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene APOE had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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