CRISPR-U™ Gene Knockout Cell Line
Strategy
IFNGR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFNGR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFNGR1 |
| Gene id | 3459 |
| Organism | Homo sapiens |
| Official full symbol | interferon gamma receptor 1 |
| Gene type | protein-coding |
| Also known as | CD119, IFNGR, IMD27A, IMD27B |
| Summary | This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFNGR1-211 | ENST00000646898.1 | 2519 | 479aa | Protein coding | CCDS87446 | A0A2R8Y4U4 | - | GENCODE basic, |
| IFNGR1-207 | ENST00000644894.1 | 2492 | 448aa | Protein coding | CCDS87445 | A0A2R8YFL3 | - | GENCODE basic, |
| IFNGR1-210 | ENST00000646036.1 | 2183 | 479aa | Protein coding | CCDS87446 | A0A2R8Y4U4 | - | GENCODE basic, |
| IFNGR1-201 | ENST00000367739.9 | 2074 | 489aa | Protein coding | CCDS5185 | P15260-1 | NM_000416.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IFNGR1-212 | ENST00000647124.1 | 1964 | 448aa | Protein coding | CCDS87445 | A0A2R8YFL3 | - | GENCODE basic, |
| IFNGR1-209 | ENST00000645753.1 | 1948 | 448aa | Protein coding | CCDS87445 | A0A2R8YFL3 | - | GENCODE basic, |
| IFNGR1-205 | ENST00000642390.1 | 2123 | 470aa | Protein coding | - | A0A2R8Y7R1 | - | GENCODE basic, |
| IFNGR1-203 | ENST00000458076.5 | 753 | 210aa | Protein coding | - | Q5TFC9 | - | CDS 3' incomplete, TSL:3, |
| IFNGR1-202 | ENST00000414770.5 | 748 | 161aa | Protein coding | - | Q5TFD1 | - | CDS 3' incomplete, TSL:3, |
| IFNGR1-206 | ENST00000643119.1 | 2136 | 30aa | Nonsense mediated decay | - | A0A2R8Y792 | - | CDS 5' incomplete, |
| IFNGR1-208 | ENST00000645045.1 | 2125 | 27aa | Nonsense mediated decay | - | A0A2R8YEN6 | - | CDS 5' incomplete, |
| IFNGR1-204 | ENST00000478333.1 | 643 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFNGR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFNGR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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