CRISPR-U™ Gene Knockout Cell Line Strategy

IFNGR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFNGR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFNGR1
Gene id 3459
Organism Homo sapiens
Official full symbol interferon gamma receptor 1
Gene type protein-coding
Also known as CD119, IFNGR, IMD27A, IMD27B
Summary This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Genomic regions Chromosome 6
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFNGR1-211 ENST00000646898.1 2519 479aa Protein coding CCDS87446 A0A2R8Y4U4 - GENCODE basic,
IFNGR1-207 ENST00000644894.1 2492 448aa Protein coding CCDS87445 A0A2R8YFL3 - GENCODE basic,
IFNGR1-210 ENST00000646036.1 2183 479aa Protein coding CCDS87446 A0A2R8Y4U4 - GENCODE basic,
IFNGR1-201 ENST00000367739.9 2074 489aa Protein coding CCDS5185 P15260-1 NM_000416.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IFNGR1-212 ENST00000647124.1 1964 448aa Protein coding CCDS87445 A0A2R8YFL3 - GENCODE basic,
IFNGR1-209 ENST00000645753.1 1948 448aa Protein coding CCDS87445 A0A2R8YFL3 - GENCODE basic,
IFNGR1-205 ENST00000642390.1 2123 470aa Protein coding - A0A2R8Y7R1 - GENCODE basic,
IFNGR1-203 ENST00000458076.5 753 210aa Protein coding - Q5TFC9 - CDS 3' incomplete, TSL:3,
IFNGR1-202 ENST00000414770.5 748 161aa Protein coding - Q5TFD1 - CDS 3' incomplete, TSL:3,
IFNGR1-206 ENST00000643119.1 2136 30aa Nonsense mediated decay - A0A2R8Y792 - CDS 5' incomplete,
IFNGR1-208 ENST00000645045.1 2125 27aa Nonsense mediated decay - A0A2R8YEN6 - CDS 5' incomplete,
IFNGR1-204 ENST00000478333.1 643 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFNGR1
This KO Strategy loading
Red Cotton™ Notes Gene IFNGR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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