CRISPR-U™ Gene Knockout Cell Line
Strategy
IFNAR2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFNAR2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFNAR2 |
| Gene id | 3455 |
| Organism | Homo sapiens |
| Official full symbol | interferon alpha and beta receptor subunit 2 |
| Gene type | protein-coding |
| Also known as | IFN-R, IFN-alpha-REC, IFNABR, IFNARB, IMD45 |
| Summary | The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family. Mutations in this gene are associated with Immunodeficiency 45. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFNAR2-205 | ENST00000404220.7 | 4284 | 331aa | Protein coding | CCDS13622 | P48551-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IFNAR2-202 | ENST00000342136.9 | 4074 | 515aa | Protein coding | CCDS13621 | P48551-1 | NM_001289125.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| IFNAR2-201 | ENST00000342101.7 | 2606 | 239aa | Protein coding | CCDS74782 | P48551-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IFNAR2-204 | ENST00000382264.7 | 1389 | 331aa | Protein coding | CCDS13622 | P48551-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IFNAR2-209 | ENST00000443073.5 | 841 | 229aa | Protein coding | - | C9JCU0 | - | CDS 3' incomplete, TSL:5, |
| IFNAR2-210 | ENST00000447980.1 | 726 | 204aa | Protein coding | - | C9JM33 | - | CDS 3' incomplete, TSL:5, |
| IFNAR2-206 | ENST00000413881.5 | 710 | 167aa | Protein coding | - | C9K067 | - | TSL:5, GENCODE basic, |
| IFNAR2-203 | ENST00000382238.6 | 1127 | 197aa | Nonsense mediated decay | - | F8WDE1 | - | TSL:3, |
| IFNAR2-207 | ENST00000417007.1 | 719 | 54aa | Nonsense mediated decay | - | H7C176 | - | CDS 5' incomplete, TSL:3, |
| IFNAR2-208 | ENST00000420068.1 | 505 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFNAR2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFNAR2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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