CRISPR-U™ Gene Knockout Cell Line Strategy

IFNAR2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFNAR2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFNAR2
Gene id 3455
Organism Homo sapiens
Official full symbol interferon alpha and beta receptor subunit 2
Gene type protein-coding
Also known as IFN-R, IFN-alpha-REC, IFNABR, IFNARB, IMD45
Summary The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family. Mutations in this gene are associated with Immunodeficiency 45.
Genomic regions Chromosome 21
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFNAR2-205 ENST00000404220.7 4284 331aa Protein coding CCDS13622 P48551-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IFNAR2-202 ENST00000342136.9 4074 515aa Protein coding CCDS13621 P48551-1 NM_001289125.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
IFNAR2-201 ENST00000342101.7 2606 239aa Protein coding CCDS74782 P48551-3 - TSL:1, GENCODE basic, APPRIS ALT2,
IFNAR2-204 ENST00000382264.7 1389 331aa Protein coding CCDS13622 P48551-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IFNAR2-209 ENST00000443073.5 841 229aa Protein coding - C9JCU0 - CDS 3' incomplete, TSL:5,
IFNAR2-210 ENST00000447980.1 726 204aa Protein coding - C9JM33 - CDS 3' incomplete, TSL:5,
IFNAR2-206 ENST00000413881.5 710 167aa Protein coding - C9K067 - TSL:5, GENCODE basic,
IFNAR2-203 ENST00000382238.6 1127 197aa Nonsense mediated decay - F8WDE1 - TSL:3,
IFNAR2-207 ENST00000417007.1 719 54aa Nonsense mediated decay - H7C176 - CDS 5' incomplete, TSL:3,
IFNAR2-208 ENST00000420068.1 505 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFNAR2
This KO Strategy loading
Red Cotton™ Notes Gene IFNAR2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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