CRISPR-U™ Gene Knockout Cell Line
Strategy
IFIT3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFIT3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFIT3 |
| Gene id | 3437 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein with tetratricopeptide repeats 3 |
| Gene type | protein-coding |
| Also known as | CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, P60, RIG-G, cig41 |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFIT3-206 | ENST00000679781.1 | 4013 | 490aa | Protein coding | CCDS7402 | Q5T765 | - | GENCODE basic, APPRIS P2, |
| IFIT3-201 | ENST00000371811.4 | 2455 | 490aa | Protein coding | CCDS31241 | O14879 Q5T765 | - | TSL:1, GENCODE basic, APPRIS P2, |
| IFIT3-202 | ENST00000371818.9 | 2390 | 490aa | Protein coding | CCDS7402 | O14879 Q5T765 | NM_001549.6 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| IFIT3-215 | ENST00000681376.1 | 3910 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-205 | ENST00000679583.1 | 2623 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-209 | ENST00000680037.1 | 2565 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-207 | ENST00000679897.1 | 2546 | 56aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-214 | ENST00000681277.1 | 2523 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-211 | ENST00000680779.1 | 2471 | 517aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| IFIT3-212 | ENST00000681178.1 | 2340 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-204 | ENST00000679536.1 | 2338 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-213 | ENST00000681207.1 | 2338 | 438aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-203 | ENST00000679438.1 | 545 | 78aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT3-208 | ENST00000679923.1 | 203 | 1aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFIT3-210 | ENST00000680085.1 | 198 | 1aa | Protein coding | - | - | - | CDS 3' incomplete, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFIT3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFIT3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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