CRISPR-U™ Gene Knockout Cell Line Strategy

IFIT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFIT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFIT1
Gene id 3434
Organism Homo sapiens
Official full symbol interferon induced protein with tetratricopeptide repeats 1
Gene type protein-coding
Also known as C56, G10P1, IFI-56, IFI-56K, IFI56, IFIT-1, IFNAI1, ISG56, P56, RNM561
Summary This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Genomic regions Chromosome 10
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFIT1-202 ENST00000546318.2 4613 447aa Protein coding CCDS59220 P09914-2 - TSL:3, GENCODE basic,
IFIT1-201 ENST00000371804.4 4302 478aa Protein coding CCDS31243 P09914-1 NM_001548.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFIT1
This KO Strategy loading
Red Cotton™ Notes Gene IFIT1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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