CRISPR-U™ Gene Knockout Cell Line
Strategy
IFIT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFIT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFIT2 |
| Gene id | 3433 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein with tetratricopeptide repeats 2 |
| Gene type | protein-coding |
| Also known as | G10P2, GARG-39, IFI-54, IFI-54K, IFI54, IFIT-2, ISG-54 K, ISG-54K, ISG54, P54, cig42 |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFIT2-203 | ENST00000638108.1 | 3703 | 472aa | Protein coding | CCDS41548 | P09913 | - | TSL:5, GENCODE basic, APPRIS P1, |
| IFIT2-212 | ENST00000680954.1 | 3643 | 472aa | Protein coding | CCDS41548 | - | - | GENCODE basic, APPRIS P1, |
| IFIT2-201 | ENST00000371826.4 | 3393 | 472aa | Protein coding | CCDS41548 | P09913 | NM_001547.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IFIT2-211 | ENST00000680809.1 | 3373 | 472aa | Protein coding | CCDS41548 | - | - | GENCODE basic, APPRIS P1, |
| IFIT2-208 | ENST00000679755.1 | 3418 | 448aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT2-207 | ENST00000679734.1 | 3382 | 448aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT2-209 | ENST00000680381.1 | 3337 | 448aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT2-202 | ENST00000611722.2 | 2497 | 200aa | Protein coding | - | A0A087X279 | - | CDS 5' incomplete, TSL:5, |
| IFIT2-206 | ENST00000679619.1 | 651 | 145aa | Protein coding | - | - | - | CDS 5' incomplete, |
| IFIT2-214 | ENST00000681843.1 | 1774 | 471aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| IFIT2-205 | ENST00000679608.1 | 484 | No protein | Processed transcript | - | - | - | - |
| IFIT2-215 | ENST00000681850.1 | 383 | No protein | Processed transcript | - | - | - | - |
| IFIT2-204 | ENST00000679606.1 | 382 | No protein | Processed transcript | - | - | - | - |
| IFIT2-210 | ENST00000680558.1 | 282 | No protein | Processed transcript | - | - | - | - |
| IFIT2-213 | ENST00000681150.1 | 263 | No protein | Processed transcript | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFIT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFIT2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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