CRISPR-U™ Gene Knockout Cell Line Strategy

IFIT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFIT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFIT2
Gene id 3433
Organism Homo sapiens
Official full symbol interferon induced protein with tetratricopeptide repeats 2
Gene type protein-coding
Also known as G10P2, GARG-39, IFI-54, IFI-54K, IFI54, IFIT-2, ISG-54 K, ISG-54K, ISG54, P54, cig42
Genomic regions Chromosome 10
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFIT2-203 ENST00000638108.1 3703 472aa Protein coding CCDS41548 P09913 - TSL:5, GENCODE basic, APPRIS P1,
IFIT2-212 ENST00000680954.1 3643 472aa Protein coding CCDS41548 - - GENCODE basic, APPRIS P1,
IFIT2-201 ENST00000371826.4 3393 472aa Protein coding CCDS41548 P09913 NM_001547.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IFIT2-211 ENST00000680809.1 3373 472aa Protein coding CCDS41548 - - GENCODE basic, APPRIS P1,
IFIT2-208 ENST00000679755.1 3418 448aa Protein coding - - - GENCODE basic,
IFIT2-207 ENST00000679734.1 3382 448aa Protein coding - - - GENCODE basic,
IFIT2-209 ENST00000680381.1 3337 448aa Protein coding - - - GENCODE basic,
IFIT2-202 ENST00000611722.2 2497 200aa Protein coding - A0A087X279 - CDS 5' incomplete, TSL:5,
IFIT2-206 ENST00000679619.1 651 145aa Protein coding - - - CDS 5' incomplete,
IFIT2-214 ENST00000681843.1 1774 471aa Nonsense mediated decay - - - CDS 5' incomplete,
IFIT2-205 ENST00000679608.1 484 No protein Processed transcript - - - -
IFIT2-215 ENST00000681850.1 383 No protein Processed transcript - - - -
IFIT2-204 ENST00000679606.1 382 No protein Processed transcript - - - -
IFIT2-210 ENST00000680558.1 282 No protein Processed transcript - - - -
IFIT2-213 ENST00000681150.1 263 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFIT2
This KO Strategy loading
Red Cotton™ Notes Gene IFIT2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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