CRISPR-U™ Gene Knockout Cell Line
Strategy
IFI35 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFI35 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFI35 |
| Gene id | 3430 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein 35 |
| Gene type | protein-coding |
| Also known as | IFP35 |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFI35-204 | ENST00000438323.2 | 1232 | 288aa | Protein coding | CCDS11450 | P80217-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| IFI35-203 | ENST00000415816.7 | 1182 | 286aa | Protein coding | CCDS82134 | P80217-1 | NM_001330230.2 | TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| IFI35-206 | ENST00000536969.1 | 383 | No protein | Processed transcript | - | - | - | TSL:3, |
| IFI35-201 | ENST00000246911.6 | 747 | No protein | Retained intron | - | - | - | TSL:2, |
| IFI35-202 | ENST00000396722.2 | 595 | No protein | Retained intron | - | - | - | TSL:2, |
| IFI35-208 | ENST00000546325.1 | 525 | No protein | Retained intron | - | - | - | TSL:5, |
| IFI35-207 | ENST00000538473.1 | 463 | No protein | Retained intron | - | - | - | TSL:3, |
| IFI35-205 | ENST00000534876.1 | 386 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFI35 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFI35 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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