CRISPR-U™ Gene Knockout Cell Line
Strategy
IFI16 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFI16 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFI16 |
| Gene id | 3428 |
| Organism | Homo sapiens |
| Official full symbol | interferon gamma inducible protein 16 |
| Gene type | protein-coding |
| Also known as | IFNGIP1, PYHIN2 |
| Summary | This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFI16-204 | ENST00000368131.8 | 2734 | 729aa | Protein coding | CCDS1180 | Q16666-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IFI16-203 | ENST00000359709.7 | 2609 | 729aa | Protein coding | CCDS58039 | Q16666-6 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| IFI16-201 | ENST00000295809.12 | 2883 | 785aa | Protein coding | - | Q16666-1 | NM_001376587.1 | TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| IFI16-202 | ENST00000340979.10 | 2732 | 243aa | Protein coding | - | A0A0A0MRB1 | - | TSL:5, GENCODE basic, |
| IFI16-205 | ENST00000368132.7 | 2704 | 729aa | Protein coding | - | Q16666-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IFI16-208 | ENST00000448393.6 | 2245 | 673aa | Protein coding | - | Q16666-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IFI16-209 | ENST00000474473.1 | 1095 | 229aa | Protein coding | - | H3BR88 | - | CDS 3' incomplete, TSL:2, |
| IFI16-207 | ENST00000447473.6 | 951 | 180aa | Protein coding | - | X6RHM1 | - | CDS 3' incomplete, TSL:5, |
| IFI16-213 | ENST00000566111.5 | 856 | 83aa | Protein coding | - | H3BVE6 | - | CDS 3' incomplete, TSL:2, |
| IFI16-206 | ENST00000426592.6 | 761 | 167aa | Protein coding | - | H3BM18 | - | CDS 3' incomplete, TSL:4, |
| IFI16-214 | ENST00000567661.5 | 546 | 74aa | Protein coding | - | H3BR65 | - | CDS 3' incomplete, TSL:4, |
| IFI16-210 | ENST00000483916.2 | 4138 | No protein | Retained intron | - | - | - | TSL:1, |
| IFI16-211 | ENST00000493884.5 | 4138 | No protein | Retained intron | - | - | - | TSL:5, |
| IFI16-212 | ENST00000562225.1 | 1108 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFI16 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFI16 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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