CRISPR-U™ Gene Knockout Cell Line Strategy

IFI16 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFI16 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFI16
Gene id 3428
Organism Homo sapiens
Official full symbol interferon gamma inducible protein 16
Gene type protein-coding
Also known as IFNGIP1, PYHIN2
Summary This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFI16-204 ENST00000368131.8 2734 729aa Protein coding CCDS1180 Q16666-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IFI16-203 ENST00000359709.7 2609 729aa Protein coding CCDS58039 Q16666-6 - TSL:2, GENCODE basic, APPRIS ALT2,
IFI16-201 ENST00000295809.12 2883 785aa Protein coding - Q16666-1 NM_001376587.1 TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92,
IFI16-202 ENST00000340979.10 2732 243aa Protein coding - A0A0A0MRB1 - TSL:5, GENCODE basic,
IFI16-205 ENST00000368132.7 2704 729aa Protein coding - Q16666-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IFI16-208 ENST00000448393.6 2245 673aa Protein coding - Q16666-3 - TSL:5, GENCODE basic, APPRIS ALT2,
IFI16-209 ENST00000474473.1 1095 229aa Protein coding - H3BR88 - CDS 3' incomplete, TSL:2,
IFI16-207 ENST00000447473.6 951 180aa Protein coding - X6RHM1 - CDS 3' incomplete, TSL:5,
IFI16-213 ENST00000566111.5 856 83aa Protein coding - H3BVE6 - CDS 3' incomplete, TSL:2,
IFI16-206 ENST00000426592.6 761 167aa Protein coding - H3BM18 - CDS 3' incomplete, TSL:4,
IFI16-214 ENST00000567661.5 546 74aa Protein coding - H3BR65 - CDS 3' incomplete, TSL:4,
IFI16-210 ENST00000483916.2 4138 No protein Retained intron - - - TSL:1,
IFI16-211 ENST00000493884.5 4138 No protein Retained intron - - - TSL:5,
IFI16-212 ENST00000562225.1 1108 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFI16
This KO Strategy loading
Red Cotton™ Notes Gene IFI16 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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