CRISPR-U™ Gene Knockout Cell Line
Strategy
IDH1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IDH1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IDH1 |
| Gene id | 3417 |
| Organism | Homo sapiens |
| Official full symbol | isocitrate dehydrogenase (NADP(+)) 1 |
| Gene type | protein-coding |
| Also known as | HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD |
| Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IDH1-203 | ENST00000415913.5 | 2441 | 414aa | Protein coding | CCDS2381 | A0A024R3Y6 O75874 | - | TSL:1, GENCODE basic, APPRIS P1, |
| IDH1-201 | ENST00000345146.7 | 2318 | 414aa | Protein coding | CCDS2381 | A0A024R3Y6 O75874 | NM_005896.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IDH1-205 | ENST00000446179.5 | 2298 | 414aa | Protein coding | CCDS2381 | A0A024R3Y6 O75874 | - | TSL:1, GENCODE basic, APPRIS P1, |
| IDH1-202 | ENST00000415282.5 | 942 | 157aa | Protein coding | - | C9J4N6 | - | CDS 3' incomplete, TSL:2, |
| IDH1-204 | ENST00000417583.5 | 702 | 73aa | Protein coding | - | C9JLU6 | - | CDS 3' incomplete, TSL:4, |
| IDH1-206 | ENST00000451391.5 | 563 | 62aa | Protein coding | - | C9JJE5 | - | CDS 3' incomplete, TSL:3, |
| IDH1-207 | ENST00000462386.5 | 1614 | No protein | Retained intron | - | - | - | TSL:2, |
| IDH1-209 | ENST00000484575.1 | 1044 | No protein | Retained intron | - | - | - | TSL:2, |
| IDH1-208 | ENST00000481557.1 | 556 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IDH1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IDH1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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