CRISPR-U™ Gene Knockout Cell Line Strategy

IDH1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IDH1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IDH1
Gene id 3417
Organism Homo sapiens
Official full symbol isocitrate dehydrogenase (NADP(+)) 1
Gene type protein-coding
Also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
Summary Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IDH1-203 ENST00000415913.5 2441 414aa Protein coding CCDS2381 A0A024R3Y6 O75874 - TSL:1, GENCODE basic, APPRIS P1,
IDH1-201 ENST00000345146.7 2318 414aa Protein coding CCDS2381 A0A024R3Y6 O75874 NM_005896.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IDH1-205 ENST00000446179.5 2298 414aa Protein coding CCDS2381 A0A024R3Y6 O75874 - TSL:1, GENCODE basic, APPRIS P1,
IDH1-202 ENST00000415282.5 942 157aa Protein coding - C9J4N6 - CDS 3' incomplete, TSL:2,
IDH1-204 ENST00000417583.5 702 73aa Protein coding - C9JLU6 - CDS 3' incomplete, TSL:4,
IDH1-206 ENST00000451391.5 563 62aa Protein coding - C9JJE5 - CDS 3' incomplete, TSL:3,
IDH1-207 ENST00000462386.5 1614 No protein Retained intron - - - TSL:2,
IDH1-209 ENST00000484575.1 1044 No protein Retained intron - - - TSL:2,
IDH1-208 ENST00000481557.1 556 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IDH1
This KO Strategy loading
Red Cotton™ Notes Gene IDH1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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