CRISPR-U™ Gene Knockout Cell Line
Strategy
ID3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ID3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ID3 |
| Gene id | 3399 |
| Organism | Homo sapiens |
| Official full symbol | inhibitor of DNA binding 3, HLH protein |
| Gene type | protein-coding |
| Also known as | HEIR-1, bHLHb25 |
| Summary | The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ID3-201 | ENST00000374561.6 | 950 | 119aa | Protein coding | CCDS237 | Q02535 | NM_002167.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ID3-203 | ENST00000486541.1 | 892 | No protein | Processed transcript | - | - | - | TSL:1, |
| ID3-202 | ENST00000463312.1 | 623 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ID3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ID3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: