CRISPR-U™ Gene Knockout Cell Line
Strategy
CCDC137 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCDC137 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCDC137 |
| Gene id | 339230 |
| Organism | Homo sapiens |
| Official full symbol | coiled-coil domain containing 137 |
| Gene type | protein-coding |
| Also known as | RaRF |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCDC137-201 | ENST00000329214.13 | 2095 | 289aa | Protein coding | CCDS42400 | Q6PK04 | NM_199287.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CCDC137-204 | ENST00000574107.1 | 890 | 292aa | Protein coding | - | I3L0U5 | - | CDS 3' incomplete, TSL:3, |
| CCDC137-206 | ENST00000575223.5 | 1888 | 289aa | Nonsense mediated decay | CCDS42400 | Q6PK04 | - | TSL:5, |
| CCDC137-202 | ENST00000571916.1 | 708 | 61aa | Nonsense mediated decay | - | I3L385 | - | CDS 5' incomplete, TSL:3, |
| CCDC137-205 | ENST00000574200.1 | 437 | 44aa | Nonsense mediated decay | - | I3L4F6 | - | CDS 5' incomplete, TSL:2, |
| CCDC137-203 | ENST00000572531.1 | 265 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCDC137 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCDC137 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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