CRISPR-U™ Gene Knockout Cell Line
Strategy
ICAM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ICAM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ICAM1 |
| Gene id | 3383 |
| Organism | Homo sapiens |
| Official full symbol | intercellular adhesion molecule 1 |
| Gene type | protein-coding |
| Also known as | BB2, CD54, P3.58 |
| Summary | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ICAM1-201 | ENST00000264832.8 | 2967 | 532aa | Protein coding | CCDS12231 | A0A384MEK5 P05362 | NM_000201.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ICAM1-202 | ENST00000423829.2 | 1296 | 310aa | Protein coding | - | E7ESS4 | - | TSL:2, GENCODE basic, |
| ICAM1-204 | ENST00000588645.1 | 581 | 180aa | Protein coding | - | K7EKL8 | - | CDS 3' incomplete, TSL:2, |
| ICAM1-203 | ENST00000585443.1 | 560 | No protein | Processed transcript | - | - | - | TSL:5, |
| ICAM1-205 | ENST00000592686.1 | 571 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ICAM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ICAM1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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