CRISPR-U™ Gene Knockout Cell Line Strategy

ICAM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ICAM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ICAM1
Gene id 3383
Organism Homo sapiens
Official full symbol intercellular adhesion molecule 1
Gene type protein-coding
Also known as BB2, CD54, P3.58
Summary This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ICAM1-201 ENST00000264832.8 2967 532aa Protein coding CCDS12231 A0A384MEK5 P05362 NM_000201.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ICAM1-202 ENST00000423829.2 1296 310aa Protein coding - E7ESS4 - TSL:2, GENCODE basic,
ICAM1-204 ENST00000588645.1 581 180aa Protein coding - K7EKL8 - CDS 3' incomplete, TSL:2,
ICAM1-203 ENST00000585443.1 560 No protein Processed transcript - - - TSL:5,
ICAM1-205 ENST00000592686.1 571 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ICAM1
This KO Strategy loading
Red Cotton™ Notes Gene ICAM1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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