CRISPR-U™ Gene Knockout Cell Line
Strategy
HTR2A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HTR2A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HTR2A |
| Gene id | 3356 |
| Organism | Homo sapiens |
| Official full symbol | 5-hydroxytryptamine receptor 2A |
| Gene type | protein-coding |
| Also known as | 5-HT2A, HTR2 |
| Summary | This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 13 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HTR2A-201 | ENST00000542664.4 | 5415 | 471aa | Protein coding | CCDS9405 | P28223-1 | NM_000621.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HTR2A-202 | ENST00000543956.5 | 4689 | 308aa | Protein coding | - | P28223-2 | - | TSL:1, GENCODE basic, |
| HTR2A-203 | ENST00000612998.1 | 509 | 110aa | Protein coding | - | A0A087WZJ9 | - | CDS 5' incomplete, TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HTR2A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HTR2A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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