CRISPR-U™ Gene Knockout Cell Line Strategy

HTR2A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HTR2A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HTR2A
Gene id 3356
Organism Homo sapiens
Official full symbol 5-hydroxytryptamine receptor 2A
Gene type protein-coding
Also known as 5-HT2A, HTR2
Summary This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 13
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HTR2A-201 ENST00000542664.4 5415 471aa Protein coding CCDS9405 P28223-1 NM_000621.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HTR2A-202 ENST00000543956.5 4689 308aa Protein coding - P28223-2 - TSL:1, GENCODE basic,
HTR2A-203 ENST00000612998.1 509 110aa Protein coding - A0A087WZJ9 - CDS 5' incomplete, TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HTR2A
This KO Strategy loading
Red Cotton™ Notes Gene HTR2A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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