CRISPR-U™ Gene Knockout Cell Line Strategy

HSP90AB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HSP90AB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HSP90AB1
Gene id 3326
Organism Homo sapiens
Official full symbol heat shock protein 90 alpha family class B member 1
Gene type protein-coding
Also known as D6S182, HSP84, HSP90B, HSPC2, HSPCB
Summary This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Genomic regions Chromosome 6
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HSP90AB1-202 ENST00000371554.2 2674 724aa Protein coding CCDS4909 A0A024RD80 P08238 - TSL:5, GENCODE basic, APPRIS P1,
HSP90AB1-204 ENST00000620073.4 2644 724aa Protein coding CCDS4909 A0A024RD80 P08238 - TSL:5, GENCODE basic, APPRIS P1,
HSP90AB1-201 ENST00000353801.7 2582 724aa Protein coding CCDS4909 A0A024RD80 P08238 - TSL:1, GENCODE basic, APPRIS P1,
HSP90AB1-203 ENST00000371646.10 2555 724aa Protein coding CCDS4909 A0A024RD80 P08238 NM_007355.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HSP90AB1
This KO Strategy loading
Red Cotton™ Notes Gene HSP90AB1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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