CRISPR-U™ Gene Knockout Cell Line
Strategy
HSP90AB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSP90AB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSP90AB1 |
| Gene id | 3326 |
| Organism | Homo sapiens |
| Official full symbol | heat shock protein 90 alpha family class B member 1 |
| Gene type | protein-coding |
| Also known as | D6S182, HSP84, HSP90B, HSPC2, HSPCB |
| Summary | This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSP90AB1-202 | ENST00000371554.2 | 2674 | 724aa | Protein coding | CCDS4909 | A0A024RD80 P08238 | - | TSL:5, GENCODE basic, APPRIS P1, |
| HSP90AB1-204 | ENST00000620073.4 | 2644 | 724aa | Protein coding | CCDS4909 | A0A024RD80 P08238 | - | TSL:5, GENCODE basic, APPRIS P1, |
| HSP90AB1-201 | ENST00000353801.7 | 2582 | 724aa | Protein coding | CCDS4909 | A0A024RD80 P08238 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HSP90AB1-203 | ENST00000371646.10 | 2555 | 724aa | Protein coding | CCDS4909 | A0A024RD80 P08238 | NM_007355.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSP90AB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSP90AB1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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