CRISPR-U™ Gene Knockout Cell Line
Strategy
HSP90AA1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSP90AA1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSP90AA1 |
| Gene id | 3320 |
| Organism | Homo sapiens |
| Official full symbol | heat shock protein 90 alpha family class A member 1 |
| Gene type | protein-coding |
| Also known as | EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2 |
| Summary | The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSP90AA1-202 | ENST00000334701.11 | 3510 | 854aa | Protein coding | CCDS32160 | P07900-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| HSP90AA1-201 | ENST00000216281.13 | 3228 | 732aa | Protein coding | CCDS9967 | P07900-1 | NM_005348.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| HSP90AA1-209 | ENST00000558600.1 | 595 | 100aa | Protein coding | - | A0A0U1RR69 | - | CDS 3' incomplete, TSL:4, |
| HSP90AA1-203 | ENST00000553585.5 | 581 | 174aa | Protein coding | - | G3V2J8 | - | CDS 3' incomplete, TSL:3, |
| HSP90AA1-204 | ENST00000554401.1 | 1710 | 52aa | Nonsense mediated decay | - | H0YJF5 | - | CDS 5' incomplete, TSL:1, |
| HSP90AA1-208 | ENST00000557234.1 | 584 | 80aa | Nonsense mediated decay | - | G3V592 | - | TSL:3, |
| HSP90AA1-207 | ENST00000557089.1 | 694 | No protein | Retained intron | - | - | - | TSL:2, |
| HSP90AA1-206 | ENST00000556554.1 | 552 | No protein | Retained intron | - | - | - | TSL:2, |
| HSP90AA1-210 | ENST00000560130.1 | 488 | No protein | Retained intron | - | - | - | TSL:2, |
| HSP90AA1-205 | ENST00000555662.1 | 429 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSP90AA1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSP90AA1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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