CRISPR-U™ Gene Knockout Cell Line Strategy

HSP90AA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HSP90AA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HSP90AA1
Gene id 3320
Organism Homo sapiens
Official full symbol heat shock protein 90 alpha family class A member 1
Gene type protein-coding
Also known as EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2
Summary The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HSP90AA1-202 ENST00000334701.11 3510 854aa Protein coding CCDS32160 P07900-2 - TSL:1, GENCODE basic, APPRIS ALT2,
HSP90AA1-201 ENST00000216281.13 3228 732aa Protein coding CCDS9967 P07900-1 NM_005348.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
HSP90AA1-209 ENST00000558600.1 595 100aa Protein coding - A0A0U1RR69 - CDS 3' incomplete, TSL:4,
HSP90AA1-203 ENST00000553585.5 581 174aa Protein coding - G3V2J8 - CDS 3' incomplete, TSL:3,
HSP90AA1-204 ENST00000554401.1 1710 52aa Nonsense mediated decay - H0YJF5 - CDS 5' incomplete, TSL:1,
HSP90AA1-208 ENST00000557234.1 584 80aa Nonsense mediated decay - G3V592 - TSL:3,
HSP90AA1-207 ENST00000557089.1 694 No protein Retained intron - - - TSL:2,
HSP90AA1-206 ENST00000556554.1 552 No protein Retained intron - - - TSL:2,
HSP90AA1-210 ENST00000560130.1 488 No protein Retained intron - - - TSL:2,
HSP90AA1-205 ENST00000555662.1 429 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HSP90AA1
This KO Strategy loading
Red Cotton™ Notes Gene HSP90AA1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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