CRISPR-U™ Gene Knockout Cell Line
Strategy
HSPB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSPB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSPB1 |
| Gene id | 3315 |
| Organism | Homo sapiens |
| Official full symbol | heat shock protein family B (small) member 1 |
| Gene type | protein-coding |
| Also known as | CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 |
| Summary | This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSPB1-201 | ENST00000248553.7 | 777 | 205aa | Protein coding | CCDS5583 | P04792 V9HW43 | NM_001540.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HSPB1-213 | ENST00000675538.1 | 812 | 174aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-218 | ENST00000676231.1 | 807 | 215aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-204 | ENST00000674547.1 | 800 | 143aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-216 | ENST00000675906.1 | 794 | 143aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-210 | ENST00000675226.1 | 776 | 162aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-206 | ENST00000674638.1 | 772 | 157aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-209 | ENST00000675134.1 | 756 | 198aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-207 | ENST00000674650.1 | 687 | 141aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB1-202 | ENST00000429938.1 | 527 | 37aa | Protein coding | - | C9J3N8 | - | TSL:1, GENCODE basic, |
| HSPB1-208 | ENST00000674965.1 | 802 | 122aa | Nonsense mediated decay | - | - | - | - |
| HSPB1-205 | ENST00000674560.1 | 436 | No protein | Processed transcript | - | - | - | - |
| HSPB1-212 | ENST00000675488.1 | 435 | No protein | Processed transcript | - | - | - | - |
| HSPB1-215 | ENST00000675733.1 | 435 | No protein | Processed transcript | - | - | - | - |
| HSPB1-214 | ENST00000675624.1 | 434 | No protein | Processed transcript | - | - | - | - |
| HSPB1-219 | ENST00000676398.1 | 434 | No protein | Processed transcript | - | - | - | - |
| HSPB1-217 | ENST00000676195.1 | 144 | No protein | Processed transcript | - | - | - | - |
| HSPB1-203 | ENST00000447574.1 | 1455 | No protein | Retained intron | - | - | - | TSL:1, |
| HSPB1-211 | ENST00000675417.1 | 1089 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSPB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSPB1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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